HPLC测定大黄、黄连药对提取物中10种成分的含量  

Determination of 10 Indicative Components in Combination Extracts of Rhubarb and Coptis chinensis by HPLC

作　　者：杨超[1] 张振秋[1] 侯学智[1] 尤春雪[1] 谢剑琳[1] 暴凤伟[1] 梁朔[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国实验方剂学杂志》2013年第7期98-102,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅课题(2008S145)

摘　　要：目的:建立高效液相色谱法测定大黄、黄连药对提取物中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、药根碱、表小檗碱、黄连碱、巴马汀、小檗碱10种成分的含量。方法:采用Phenomsil BDS C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸(65∶35),检测波长254 nm;乙腈-水(37∶63,内含磷酸二氢钾0.34 g,十二烷基硫酸钠0.17 g),检测波长345 nm,柱温25℃。结果:大黄中5种有效成分完全分离;黄连中5种有效成分完全分离;峰面积与其质量浓度呈良好的线性;加样回收率(n=6)分别为100.79%,99.38%,99.83%,98.94%,98.86%,100.20%,99.53%,99.76%,100.07%,100.37%。RSD分别为1.3%,1.8%,1.6%,0.5%,1.6%,1.2%,1.6%,1.8%,2.0%,1.6%。结论:该方法准确可靠、重复性好,可用于大黄、黄连药对提取物的质量控制。Objective ： To develop an HPLC method for determination of 10 indicative components （aloe- emodin, rhein, emodin, chrysophanol, physcion, jatrorrhizine, epiberberine, coptisine, palmatine, berberine） in Rhubarb and Coptis chinensis. Method： Phenomsil BDS CIs （4.6 mmx 250 ram, 5 txm） was adopted; the mobile phase was methanol-0. 1% phosphoric acid （65： 35）. The detection wavelength was set at 254 nm; the other mobile phase was acetonitrile-water （37： 63） with 0.34 g potassium phosphate and 0. 17 g SDS; the detection wavelength was set at 345. The column temperature was maintained at 25℃. Result： The complete separation was bbtained for the five compounds of Rhubarb. The complete separation was obtained for the five compounds of Coptis ehinensis. Ten regression equation showing linear relationships between peak area and content of each compound were obtained. The average recoveries （n = 6） of the compounds listed above were 100.79%, 99.38%, 99. 83%, 98.94%, 98.86%, 100.20%, 99.53%, 99.76%, 100.07%, 100.37% and RSDs were 1.3% 1.8%, 1.6%, 0.5%, 1.6%, 1.2%, 1.6%, 1.8%, 2.0%, 1.6%, respectively. Conclusion： This method is dependable, simple and practical, and may be used to control quality of Rhubarb and Coptis chinensis.

关 键 词：大黄 黄连 大黄黄连泻心汤 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]