炉内消解-石墨炉原子吸收法测定明胶空心胶囊中铬的含量  

Determination of Chromium in Gelatin Empty Capsules by Furnace Digestion-AAS

作　　者：刘童[1,2] 王爱民[1,2] 关焕玉[1,2] 周雯[1,2] 张洁[1,2] 李勇军[1,2] 

机构地区：[1]贵阳医学院药学院,贵阳550004 [2]贵州省药物制剂重点实验室,贵阳550004

出　　处：《中国实验方剂学杂志》2013年第7期108-112,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化专项基金项目(黔科合中药字[2011]5081号);贵阳市科学技术计划项目([2009]筑科中合同字第9-3-04号);省科技厅科技计划项目(黔科合计工字[2009]4001)

摘　　要：目的:建立一种石墨炉炉内消解-原子吸收光谱法测定明胶空心胶囊中铬含量测定方法,并与微波消解方法比较。方法:分别将明胶空心胶囊用微波消解后的溶液与水直接溶解后的溶液注入原子吸收光谱仪的石墨炉中,采用不同的升温程序测定铬的含量。结果:两种方法铬元素浓度在4～40μg.L-1时线性关系良好,相关系数均为0.999 9,炉内消解直接进样方法的回收率为96.2%～101.2%,RSD 1.8%～3.3%,微波消解方法的回收率为97.51%～103.1%,RSD 3.4%～5.3%,炉内消解直接进样的供试品溶液在4 h以内稳定。结论:石墨炉炉内消解-原子吸收光谱法与微波消解方法测定的结果一致,该方法简便、准确,快速,适合大批量明胶空心胶囊中铬含量超标的筛查。Objective： To establish a method for determination of chromium in gelatin empty capsules which were digested in furnace by AAS. Method： The solution of gelatin empty capsules which digested by microwave or dissolved directly with water was injected into the graphite furnace of AAS, and determined the content of chromium with different temperature-programmed. Result： The calibration curves of both methods for chromium standard solution were prepared covering the concentration ranges of 4 to 40μg -L-1. The correlation coefficients were all 0. 999 9. The average recoveries of furnace digestion were in range of 96.2% to 101.2% and the RSD were 1.8% to 3.3%. The average recoveries of microwave digestion were in range of 97.51% to 103.1% and the RSD was 3.4% to 5.3%. The sample solution which would be injected directly and digested in furnace was stable within 4 hours. Conclusion： The results which determined by graphite furnace digestion and microwave digestion were consistent. This new method is easy, accurate and suitable for screening the exceeded content of chromium in the large quantities of gelatin empty capsules.

关 键 词：明胶空心胶囊 石墨炉-原子吸收光谱 炉内消解 铬 

分 类 号：R284.1[医药卫生—中药学]