丁基苯酞对大鼠弥漫性脑创伤微循环障碍的改善作用  

Protection Effects of dl-3n-butyphthalide against Cerebral Microcirculation Dysfunction Induced by Severe Diffuse Brain Injury in Rats

作　　者：赵雅宁[1] 牛静[1] 李建民[1] 薛承景[1] 陈长香[1] 李淑杏[1] 

机构地区：[1]河北联合大学康复医学院,河北唐山063000

出　　处：《中国实验方剂学杂志》2013年第7期203-207,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省科技厅支撑课题(20276102D);河北省教育厅重点课题(ZD2010106)

摘　　要：目的:研究丁基苯酞对大鼠弥漫性脑创伤微循环障碍的作用。方法:135只雄性SD大鼠随机分为假手术组(21只)、模型组(38只)、丁基苯酞高(38只)、低剂量组(38只)(80,160 mg.kg-1),治疗组在致伤后采用腹腔注射,每24 h 1次,连续注射72 h。采用Marmarou's法建立大鼠弥漫性颅脑损伤模型。术后24,48,72 h应用激光多普勒血流计观察脑组织血流量变化;宁酸-氯化铁媒染标记微血管密度,电镜观察脑组织超微结构变化,并对大鼠神经运动功能和综合运动能力评分。结果:与假手术组比较,模型组24,48,72 h脑血流量显著降低(P<0.05),血管密度显著降低(P<0.05),神经元内细胞器、轴索及毛细血管等超微结构的损伤明显,神经功能与综合运动能力评分明显下降;与创伤组比较,丁基苯酞组24,48,72 h脑血流血量增加(P<0.05);血管密度显著增加(P<0.05);神经元内细胞器、轴索及毛细血管等超微结构的损伤程度明显减轻;神经功能与综合运动能力评分明显显著回升(P<0.05),上述变化在高剂量组显著。结论:研究表明丁基苯酞可改善脑创伤后神经功能损伤,其机制可能与微循环障碍损害有关。Objective： To investigate the therapeutic effects of dl-3n-butyphthalide （NBP） on neuromotor function and its mechanism of microcirculation dysfunction following diffuse brain Injury （DBI） in rats. Method： One hundred and thirty-five male SD rats were randomly divided into sham group （21） , model group （38）, low-dose NBP treatment group （38） and high-dose NBP treatment group （38）. Treatment groups were adiministrated after injury by intraperitoneal injection, once for 24 h, continued to 72 h rat diffuse brain injury model was established by using Marmarou＇s method. The laser Doppler flowmeter was used to assay brain tissue changes of blood flow after 24, 48, 72 h tannic acid-ferric chloride mordant staining was used to determine the marking microvessel density, brain tissue changes of morphous was measured by electron microscopy; behavioral tests were performed. Result： Compared with sham group, brain tissue was damaged, the cerebral blood flows decreased at 24, 48, 72 h. The neuroscores and the general movement ability were damaged in model groups. Compared with model group, the damage of brain tissue was decreased, the cerebral blood flows increased at 24, 48, 72 h （P 〈 0. 05）. The vascular density increased （P 〈 0.05）. The neuroscores and the general movement ability enhanced in NBP groups. The above mentioned indexes changed more significantly in high dose of NBP group. Conclusion： NBP can improve neurological function after brain trauma injury, which is in part through attenuating the cerebral microcirculation damage.

关 键 词：弥漫性脑创伤 脑血流 微循环 

分 类 号：R285.5[医药卫生—中药学]