苗药迷沉方对血管性痴呆大鼠海马内VECF,flt-1,BDNF及bFGF表达的影响  

Effects of Miao Medicine Michen Decoction on Expressions of Hippocampal VECF,flt-1,BDNF,and bFGF in Vascular Dementia Rats

作　　者：张贵源[1] 胡建山[2] 李溥[2] 黄月娜[3] 何江[2] 李凡益[2] 何蕾[2] 刘毅[2] 

机构地区：[1]黔南民族医学高等专科学校外科教研室,贵州都匀558001 [2]黔南民族医学高等专科学校第二附属医院神经内科,贵州都匀558000 [3]黔南民族医学高等专科学校第二附属医院检验科,贵州都匀558000

出　　处：《中国实验方剂学杂志》2013年第7期237-241,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局苗医苗药重点研究专项基金(ZDYJSA201102)

摘　　要：目的:观察苗药迷沉方对血管性痴呆(VD)大鼠海马组织血管内皮生长因子(VEGF)、fam样酪氨酸激酶-1(flt-1)、脑源性神经营养因子(BDNF)和碱性成纤维细胞生长因子(bFGF)表达的影响,探讨苗药迷沉方对VD脑保护作用机制。方法:选取98只Wistar大鼠,采用双侧颈动脉永久结扎术制备VD模型,电脑随机数字表法随机分为:模型组、西药组、苗药迷沉方高、中、低剂量组,并设假手术组对照。假手术组及模型组给予蒸馏水灌胃(6 mL.kg-1);西药组采用盐酸多奈哌齐(0.62mg.kg-1)蒸馏水混合液灌胃;苗药组给予苗药迷沉方低(16 g.kg-1)、中(32 g.kg-1)、高(64 g.kg-1)剂量灌胃;4周为1个疗程,共治疗2个疗程。神经学评分并结合Morris迷宫实验检测造模成功后4 d、治疗8周后学习记忆成绩;采用酶联免疫吸附法检测大鼠海马组织VEGF,flt-1,BDNF和bFGF表达水平。结果:经8周治疗后,动物逃避的潜伏期、错误次数及神经行为学评分,苗药迷沉方高、中、低剂量组及西药组与模型组比较差异有统计学意义(P<0.05,P<0.01),苗药迷沉方中剂量组潜伏期、错误次数及神经行为学评分与西药组比较,差异有统计学意义(P<0.01)。苗药迷沉方、西药在提高VD大鼠潜伏期,降低VD大鼠错误次数以及对神经行为学评分影响均有显著疗效,而苗药迷沉方组明显优于西药组。苗药迷沉方高、中、低剂量组及西药组与模型组比,海马内表达VEGF,flt-1,BDNF及bFGF含量水平,差异均有统计学意义。苗药迷沉方中剂量组海马内表达VEGF,flt-1,BDNF及bFGF含量升高最为明显。结论:苗药迷沉方能改善VD大鼠行为学评分及提高记忆成绩,其机制可能为增强VD大鼠海马内VECF,flt-1,BDNF及bFGF的表达水平,激发脑内神经的损伤修复达到发挥脑保护作用。Objective： To observe the expression levels of hippocampal vascular endothelial growth factor （VECF） , fins-like tyrosine kinase-1 （fit-1） , brain-derived neurotrophic factor （BDNF） , and basic fibroblast growth factor （bFGF） in vascular dementia rats, thus studying the angiogenesis mechanism of moxibustion in vascular dementia （VD）. Method： Ninty-eight rats were selected. The VD model was established by permanent bilateral occlusion of both common carotid arteries （2-VO）. Then the modeled rats were rando mLy divided into 5 groups, ie, the memory deficit model group, the donepezil group, and the positive drug control groups [ including high （n = 14） , middle （ n = 14）, and low dose （n = 14） Miao medicine Michen decoction group. Besiders, another 14 rats were chosen as the sham-operative group. The distilled water was given by gastrogavage to rats in the sham-operative group and the memory deficit model group （6 mL. kg-1）. The donepezil hydrochloride suspension was given to rats in donepezil group by gastrogavage （0.62 mg·kg-1）. High （64 g·kg-1） , middle （32 g·kg-1）, and low （16 g·kg-1） dose of Miao medicine Michen decoction were respectively given to rats in the other three groups for 2 theapeutic courses, 4 weeks as one course. The learning and memory results were observed by the neuroethological score in morriswater maze experiment before treatment and by the end of the 2 course respectively. The expression levels of hippocampal VECF, fit-l, BDNF, and bFGF of all rats were detected using enzyme linked immunosorbent assay. Result： After 2 courses of treatment, statistical difference existed in the latent period, the error times, and the neuroethological score in the high, middle, and low dose of Miao medicine Michen decoction groups and the western medicine group when compared the model group （P 〈 0.05 or P 〈 0.01 ）. statistical difference existed in the latent period and the neuroethological score between the middle dose of Miao medicine

关 键 词：苗药迷沉方 血管性痴呆 血管内皮生长因子 fam样酪氨酸激酶-1 脑源性神经营养因子 碱性成纤维细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]