降脂合剂对高脂血症大鼠LDL-C氧化易感性及肝细胞LDL-R基因表达的影响  

Effect of Jiangzhi Mixture on LDL Susceptibility to Oxidation and Liver Cells LDL-R Gene Expression of Hyperlipidemia Rats

作　　者：谢慧臣[1] 刘芬[1] 田春漫[1] 向靖[2] 杨强[2] 

机构地区：[1]湖北民族学院医学院中西医结合教研室,湖北恩施445000 [2]湖北民族学院医学院附属医院中西医结合科,湖北恩施445000

出　　处：《中国实验方剂学杂志》2013年第7期245-249,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省自然科学基金项目(2011ABA156)

摘　　要：目的:探讨降脂合剂对高脂血症大鼠低密度脂蛋白(LDL)氧化易感性及肝细胞低密度脂蛋白受体(LDL-R)基因表达的影响,探讨降脂合剂调节血脂的可能机制。方法:将雄性Wistar大鼠60只随机分为正常组,模型组,辛伐他汀组(7.2×10-4g.kg-1)和降脂合剂0.25,0.5,1.0 g.kg-1剂量组,每组10只动物。除正常组用基础饲料喂养外,其余各组喂饲高脂饲料建立高脂血症大鼠模型,造模同时预防给药,每日1次,给药10周。10周后动物眼底静脉取血,全自动生化分析仪测定大鼠血脂,分离LDL,体外测定其氧化易感性。Trizol法提取大鼠肝脏RNA,RT-PCR测定LDL-R mRNA的表达。结果:与模型组比较,降脂合剂各剂量组预防用药后,胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和载脂蛋白B(ApoB)的水平明显降低,高密度脂蛋白(HDL-C)、载脂蛋白A(Apo A)的水平及Apo A/Apo B均有不同程度的升高,差异均有统计学意义(P<0.05或P<0.01);与模型组比较,降脂合剂各剂量组最大氧化速率时间(Tmax)和LDL氧化延迟时间(lag time)均明显延长,肝脏LDL-R mRNA相对表达量明显升高,差异均有统计学意义(P<0.05或P<0.01)。结论:降脂合剂能够调节实验性高脂血症大鼠血脂代谢,明显降低高脂血症大鼠LDL氧化易感性,促进高脂血症大鼠肝组织LDL-R mRNA基因表达,对血脂异常有较好的调节作用。Objective： To study the effect of Jiangzhi Mixture （IM） on low density lipoprotein （LDL） oxidative susceptibility and low density lipoprotein receptor （LDL-R） gene expression in rats with hyperlipidemia, and eiucidate the possible mechanism of IM regulating blood lipid. Method： Sixty Wistar male rats were randomly divided into normal group, model group, simvastatin and IM high, middle, low dose groups, 10 rats in each group. The rats in normal control group were fed with basal diet, the rats in the other groups were fed with a high fat diet to establish hyperlipidemia rat models, and during modeling the drugs were used for prevention. Physiological saline were given to the rats in control group and model group, simvastatin group rats were given 7. 2 x 10-4g .kg-1 simvastatin suspension solution, and Jiangzhi Mixture low, middle, and high dose groups were intragastrically given 0. 25, 0. 5, 1 g·kg-1 decoction, respectively, 10 mL·kg-1 , once a day for 10 weeks, during making model. After 10 weeks, according to the method of abstracting eyeball blood, the blood lipid spectrum of each individual animal in various groups were measured with full automatic biochemical analyzer. After separation of LDL, its susceptibility to oxidation was determinated in vitro. According to the method of Trizol extracting liver RNA of rats, LDL-R mRNA expression was determinated by RT-PCR. Result： Compared with model group, the cholesterol （TC）, triglycerides （TG）, low density lipoprotein chelesterol （LDL-C）, and apolipoprotein B （Apo B） levels in Jiangzhi Mixture groups were regulated and inhibited; the high density lipoprotein chelesterol （HDL-C）, apolipoprotein A （Apo A） levels and Apo A/Apo B ratio were increased, meanwhile the differences were statistically significant （P 〈 0. 05 or P 〈 0. 01 ） ; Compared with the model group, the maximum rate of oxidation time （ Tmax ） and LDL oxidation lag time （lag time） were significantly prolonged, and the relative expression levels o

关 键 词：降脂合剂 高脂血症 血脂谱 低密度脂蛋白 低密度脂蛋白受体 基因 

分 类 号：R285.5[医药卫生—中药学]