大黄庶虫虫丸对动脉粥样硬化鼠NF-κB通路及炎性因子影响  

Effects of Dahuang Zhechong Pill on the Expression of NF-κB Pathway and Inflammatory Factors in Atherosclerosis Rats

作　　者：司秋菊[1] 张艳慧[1] 王鑫国[1] 江玉娟[2] 王贵娟[1] 

机构地区：[1]河北医科大学,石家庄050091 [2]沧州市中心医院感染科,河北沧州061001

出　　处：《中国实验方剂学杂志》2013年第7期254-258,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省教育厅项目(20042011);河北省中医管理局项目(05163)

摘　　要：目的:观察大黄庶虫虫丸(DHZCP)对动脉粥样硬化(AS)大鼠炎性相关因子表达的影响,探讨DHZCP抗AS免疫炎症的机制。方法:SD雄性大鼠32只随机分为对照组、模型组、DHZCP高剂量、低剂量(1.4,0.7 g.kg-1)组,每组8只。采用高脂饲喂联合维生素D3(VitD3)复制大鼠AS模型,21 d后,用DHZCP灌胃8周,观察各组大鼠甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)及血清肿瘤坏死因子(TNF-α),HE染色观察主动脉病理变化,免疫组织化学染色观察核因子-κB(NF-κB)及细胞间黏附分子(ICAM-1)蛋白表达,并采用半定量计算机图像分析计算NF-κB及ICAM-1阳性细胞率。结果:模型组血脂、血清TNF-α升高;主动脉内膜显著增厚,可见纤维帽和胆固醇结晶;NF-κB及ICAM-1蛋白表达阳性细胞率分别是(47.35±5.18)%,(55.92±0.40)%,明显高于DHZCP治疗组(P<0.01,P<0.05)。DHZCP 2个剂量组血清脂质、TNF-α明显低于模型组;DHZCP高剂量组主动脉病变较低剂量组轻,明显优于模型组;NF-κB及ICAM-1蛋白表达阳性细胞率(16.71±4.43)%,(19.73±0.28)%,明显低于模型组(P<0.01,P<0.05)。结论:DHZCP通过下调NF-κB信号通路的蛋白表达,减少TNF-α,ICAM-1等炎性因子释放,抑制炎症反应,发挥抗AS作用。Objective： This experiment is mainly to estimate the expression nuclear factor-kappa B and inflammatory factors related in atherosclerosis （AS） model rats and investigate the anti-AS mechanism of Dahuang Zhechong Pill （DHZCP）. Method： SD rats were divided into control group, model group, high and low dose DHZCP treated group （ 1.4, 0.7 g·kg-1 ）. There are average eight rats every group. AS model was made by high fat feeding and injected vitamin D3. After 21 days, low and high dose DHZCP treated group were given low and high dose DHZCP respectively except control group and model group. Rats were fed totally for 8 weeks, the level of serum total cholesterol （TC）, triglyceride （TG）, highdensity lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, tumor necrosis factor （TNF-a）, pathological change of aortic tissue were observed. Immunohistochemical technique was utilized to measure the expression of nuclear factor KB （NF-KB） and intercellular adhesion molecule-1 （ICAM-1）. Positive cells rate of NF-KB and ICAM-1 made by half quantitative computer image analysis. Result： Compared with control group, the level of TC, TG, LDL-C, TNF-a in model group were higher and HDL-C was lower remarkably. HE staining revealed the aortic intima was obviously thickened. Cholesterol crystal and fibrous cap were seened in the aortic intima plaque. Immunohistochemical detection showed NF-KB and ICAM-1 protein were found in the intima plaque and plasma membrane of foam cells in model group, While the positive-cells rate of NF-KB and ICAM-1 expression were respectively （47.35±5.18） % and （55.92±0.40） % increased than treatment group （P 〈 0. 01, P 〈 0.05 ）. Compared with model group, the level of TC, TG, LDL-C, TNF-a in two treatment group were lower and HDL-C was higher remarkably （P 〈0.01, P 〈 0.05）. Pathological change in high dose DHZCP group is most remarkably decreased than others, While in low dose DHZCP is better than model group. The po

关 键 词：动脉粥样硬化 核因子-ΚB 大黄庶虫虫丸 肿瘤坏死因子 细胞间黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]