高良姜总黄酮对大鼠内脏高敏感性模型及镇痛作用研究  

Effect of Total Flavonoids from Alpinia officinarum on Visceral Hypersensitivity in Rat Model with Irritable Bowel Syndrome and Analgesic Effects

作　　者：梁万年[1] 江涛[2] 陈艳芬[1] 冯毅凡[3] 楚博[1] 范华倩[1] 唐春萍[1] 

机构地区：[1]广东药学院中药学院,广州510006 [2]广东药学院实验动物中心,广州510006 [3]广东药学院实验中心,广州510006

出　　处：《中国实验方剂学杂志》2013年第7期263-267,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省教育部产学研结合项目(2006D90504006);广东省自然科学基金项目(S2012040006768)

摘　　要：目的:研究高良姜总黄酮(GLJ)对乙酸诱导内脏高敏性肠易激综合征(IBS)模型大鼠的治疗作用及其镇痛作用。方法:建立大鼠IBS模型,将实验动物随机分为模型组、匹维溴铵组(0.02 g.kg-1)、GLJ高、中、低剂量组(1,0.5,0.25 g.kg-1),连续给药2周后评估乙酸诱导IBS模型大鼠的腹部回缩反射(AWR)状态,观察GLJ对乙酸诱导IBS模型大鼠的治疗作用;采用热板法、乙酸扭体法、压尾法、甲醛法研究GLJ的镇痛作用。结果:GLJ能够有效降低乙酸诱导IBS模型大鼠内脏的敏感性(P<0.01或P<0.05);对热刺激、乙酸、甲醛所诱发的小鼠疼痛均有一定的抑制作用(P<0.01或P<0.05)。结论:GLJ对IBS模型大鼠有较好的治疗作用及镇痛作用。Objective： To study the therapeutic effect in visceral hypersensitivity rat model with irritable bowel syndrome （IBS） induced by acetic acid and analgesic effect of total flavonoids from Alpinia officinarum （GLJ） and supply experimental evidence to the therapy of irritable bowel syndrome. Method： IBS models in SD rats were established by acetic acid for 2 weeks, and then the rats were randomized into model group, pinaverium group, GLJ high dose group, GLJ middle dose group, and GLJ low dose group. After treatment for 2 weeks, the therapeutic effect of GLJ was assessed by observing the abdominal withdrawal reflex （AWR） condition. The analgesic effects of GLJ were observed in pain models of mice induced by hot plate, acetic acid, pressure and formalin. Result： GLJ could effectively reduce the sensitivity of visceral hypersensitivity rat model of IBS induced by acetic acid （P 〈0. 01 or P 〈0. 05） and had analgesic effect on the pain models of hot plate, acetic acid and formalin （P 〈 0.01 or P 〈 0.05）. Conclusion： GLJ has better therapeutic effect on visceral hypersensitivity rat model of IBS and analgesic.effect.

关 键 词：高良姜总黄酮 肠易激综合征 内脏高敏性 大鼠模型 镇痛 

分 类 号：R285.5[医药卫生—中药学]