维生素E对氯氰菊酯致小鼠肺组织氧化损伤的拮抗  

Antagonism of vitamin E to oxidative damages induced by cypermethrin in lung tissue among mice

作　　者：马萍[1,2] 周慧芬[1] 陈姣娥[1] 武阳[2] 陈绍恢[2] 杨旭[2] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100 [2]华中师范大学生命科学学院环境生物医学实验室

出　　处：《环境与健康杂志》2013年第3期207-210,F0003,共5页Journal of Environment and Health

基　　金：湖北省教育厅科学技术研究项目(D20122803)

摘　　要：目的研究维生素E对氯氰菊酯致小鼠肺组织氧化损伤的拮抗。方法雄性昆明小鼠随机分为6组,包括1个阴性对照(花生油)组、3个氯氰菊酯(10、20和40 mg/kg)染毒组、1个维生素E(100 mg/kg)组和1个高剂量氯氰菊酯(40 mg/kg)加维生素E(100 mg/kg)组,灌胃7 d,测定肺组织匀浆活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)的含量和肺组织细胞DNA-蛋白质交联(DPC)系数;并观察病理损伤。结果随着氯氰菊酯染毒剂量的升高,肺组织的脏器系数、ROS、MDA含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系。除20 mg/kg组的MDA、DPC系数外,20、40 mg/kg组上述指标与对照组差异均有统计学意义(P<0.05,P<0.01)。病理学观察可见20、40 mg/kg组肺泡壁血管扩张充血,肺泡壁增厚,肺泡组织血管扩张充血。与高剂量染毒组相比较,高剂量染毒加维生素E组肺组织的脏器系数、ROS、MDA含量和DPC系数均有下降,GSH含量上升(P<0.05,P<0.01)。结论较高剂量(20、40 mg/kg)的氯氰菊酯能造成小鼠肺组织的氧化损伤和病理损伤,且可以被维生素E的抗氧化作用所拮抗。Objective To evaluate the oxidative damage effects of cypermethrin on mouse lung tissue cells and antagonism of vitamin E. Methods Kunming mice were randomly divided into six groups： one solvent control group （peanut oil）, three cypermethrin groups （10 mg/kg, 20 mg/kg and 40 mg/kg group）, one high dose cypermethrin （40 mg/kg） plus vitamin E （100 mg/kg）protection group and one vitamin E group （100 mg/kg）. The treatment was conducted through gavage, once a day for one week. Afterwards, the biomarkers of reactive oxygen species （ROS）, glutathione （GSH）, malondialdehyde（MDA） and DNA-protein crosslinks （DPC） coefficients in the lung tissues were detected, and the pathological examination was conducted. Results The levels of ROS, MDA, organ coefficients, and DPC coefficients in lung increased gradually in a dose- dependent manner, whereas GSH content decreased accordingly. Except the DPC coefficients and MDA in 20 mg/kg group, other biomarkers were significantly higher than those of the control group （P〈0.05, P〈0.01）. Furthermore, the dilated congestion had become more severe, and so was the thickening of the alveolar wall. Administration of vitamin E to cypermethrin -treated mice reflected that organ coefficients, ROS, MDA and DPC increased but GSH decreased （P〈0.05, P〈0.01）. Conclusion Cypermethrin at certain doses （20 mg/kg and 40 mg/kg） can induce oxidative stress and pathological damages in mice lung tissue, while vitamin E can against the damage induced by cypermethrin.

关 键 词：氯氰菊酯 活性氧 还原型谷胱甘肽 丙二醛 DNA-蛋白质交联 氧化损伤 

分 类 号：R994.6[医药卫生—毒理学]