妊娠期肝内胆汁淤积症胎盘合体滋养细胞中白细胞介素18和半胱氨酸天冬氨酸蛋白酶-1表达及其意义  

Expression of IL-18 and caspase-1 in placental syncytiotrophoblast of intrahepatic cholestasis of pregnancy.

作　　者：黄婷[1] 罗红霞[1] 邵勇[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《中国实用妇科与产科杂志》2013年第4期279-283,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：重庆市科委自然科学基金(CSTC2007BB5278);重庆市卫生局基金(2009-2-386)

摘　　要：目的通过检测妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)孕妇胎盘合体滋养细胞中白细胞介素18(IL-18)和半胱氨酸天冬氨酸蛋白酶-1(caspase-1)的定位和表达及caspase-1的生物学活性,探讨其与ICP发病的关系。方法 2011年3月至11月在重庆医科大学附属第一医院选择ICP孕妇40例为研究对象,包括ICP轻度组(20例),ICP重度组(20例),以同期行择期剖宫产术的20例正常孕妇为对照组。采用免疫组化检测IL-18和caspase-1的表达。结果 ICP重度组孕妇血清中IL-18及丙氨酸转氨酶、天冬氨酸转氨酶水平显著高于轻度组和正常对照组,差异有统计学意义(P<0.05)。ICP重度组胎盘合体滋养细胞中IL-18和caspase-1表达水平显著高于轻度组和正常对照组,差异有统计学意义(P<0.05)。结论 IL-18和caspase-1信号通路介导的免疫损伤可能参与了ICP孕妇的肝功能损害。Objective To evaluate the expression and location of the Interleukin-18 （IL-18）, cysteinyl aspartate-specifi proteases-1 （ caspase-1 ） and the activity of caspase-1 in human placenta syncytiotrophoblast and explore the relationship between IL-18 and caspase-1 in intrahepatic cholestasis of pregnancy（ICP）. Methods Between Mar. 2011 and Nov.2011 in the First Affiliated Hospital of Chongqing Medical University. Forty women with ICP were chosen as the study ob jeers. They were classified into mild group （n = 20）, severe group （n = 20 ）. Twenty healthy pregnant women who deliv ered in the same period were chosen as the control group （n = 20）. Results The serum level of IL-18, ALT, AST in the severe ICP group were significantly higher than the mild ICP group and control group （P 〈0. 05 ）. The IL-18 an（ caspase-1 protein levels in placenta syncytiotrophoblast of the severe ICP group were significantly higher than the mild ICP group and control group （P 〈 0. 05）. Conclusion The immune injury mediated by IL-18 and caspase-1 signaling path ways may participate in the pathogenesis of ICP hepatic damage process.

关 键 词：妊娠期肝内胆汁淤积症 合体滋养细胞 白细胞介素18 半胱氨酸天冬氨酸蛋白酶-1 

分 类 号：R714.255[医药卫生—妇产科学]