巨细胞病毒对小鼠心脏移植术后急性排斥反应的影响  

Influence of MCMV on acute rejection in mice heterotopic cardiac transplantation

作　　者：彭润生[1] 陈昊[1] 杨兆华[1] 王春生[1] 

机构地区：[1]复旦大学附属中山医院心外科上海市心血管病研究所复旦大学器官移植中心,上海200032

出　　处：《中国实验诊断学》2013年第3期425-428,共4页Chinese Journal of Laboratory Diagnosis

基　　金：上海市重点学科建设项目(B116)

摘　　要：目的观察巨细胞病毒(cytomegalovirus,CMV)感染对同种异基因小鼠腹腔异位移植心脏急性排斥反应的影响。方法 BALB/c小鼠80只,C57BL/6小鼠40只,按供、受鼠基因不同分为3组(每组20对):同质移植组(A组,BALB/c→BALB/c)、环胞素A组(B组,C57BL/6→BALB/c)和小鼠巨细胞病毒联合环胞素A组(C组,C57BL/6→BALB/c),施行小鼠腹腔异位心脏移植术。A组无药物干预,B组术后腹腔注射CsA 20mg/kg.d,C组除腹腔注射CsA 20mg/kg.d外,术后第1d腹腔接种104PFU小鼠巨细胞病毒(MCMV)0.2ml。术后观察移植鼠心脏跳动情况、移植心脏存活时间、移植心脏的病理组织学以及MCMV病毒滴度测定。结果术后10d,A组移植心脏几乎无排斥反应;B组移植心脏搏动有力,显微镜下观察移植心脏心肌间质内有局灶性炎症细胞浸润,心肌有变性;C组移植心脏搏动微弱,体积增大,重量增加,显微镜下见心内、外膜下及心肌间质有弥漫性淋巴细胞及单核细胞浸润;心肌细胞可发生变性、坏死。C组(12.4±1.3d)移植心脏存活时间明显低于B组(16.7±1.9d)(P<0.01),而A组移植心脏长期存活(观察60d)。结论巨细胞病毒感染加速小鼠腹腔异位移植心脏急性排斥反应。Objective To observe the influence of MCMV infection on acute rejection in mice heterotopic allograft cardiac transplantation. Methods One hundrad and twenty mice were randomly divided into three groups： isograft group （group A,BALB/c→BALB/c）,allograft CsA group （group B,C57BL/6-→BALB/c） and allograft CsA ＋MCM- Vgroup（group C,C57BL/6→BALB/c）. CsA 20 mg/kg, d was given in group B and group C i. p while 104PFI） MCMV 0.2 ml i. p in group C at first day after postoperation,and same dosage volumn of 0.9% NS was given in group A. The heat beating and survival time of the transplanted hearts and pathology at 10th postoperation were observed. Results At 10d after operation, the rejection was not observed in the group A;in the group B,the transplanted heats were beat- ing powerful,and the inflamation cells in local were found in the myocardial interstitium, and myocardial degeneration was appeared; in the group C, the transplanted heats were beating weak, the size and weight of the hearts increased. Lymphocytes and monocytes were infiltrated diffuse in endocardium, epicardium and myocardial interstitium, myocardial degeneration and necrosis was appeared. Furthermore, the survival time of the transplanted hearts were longer in group B （16.7±1.9 d） than that in group C （12.4±1. 3 d） （P〈0.01）. In the group A,the transplanted heats were alive un- til the last visit. （60 d） Conclusion MCMV infection accelerates allograft acute rejection in mice heterotopic cardiac transplantation.

关 键 词：巨细胞病毒 加速 急性排斥 心脏移植 

分 类 号：R654.2[医药卫生—外科学]