大菱鲆子二代家系及亲本耐高温能力比较  

Comparison of high temperature tolerance of turbot Scophthalmus maximus F_2 families and broodstock

作　　者：关健[1] 刘梦侠[1] 刘洪军[1] 于道德[1] 官曙光[1] 郑永允 

机构地区：[1]山东省海水养殖研究所,青岛266002

出　　处：《渔业科学进展》2013年第1期103-110,共8页Progress in Fishery Sciences

基　　金：山东省农业良种工程"优质抗病速生鱼类良种选育-工厂化适养品种的选育";国家鲆鲽类产业技术体系建设项目(nycytx-50)共同资助

摘　　要：通过高温刺激和高温水养殖幼鱼的方法研究了大菱鲆不同子二代(F2)家系耐高温能力。对2010年建立的36个子二代(F2)家系50日龄幼鱼,记录使用30℃高温海水进行突变热刺激2h后48h的死亡率;对2009年和2010年所建立的22个和36个大菱鲆F2家系100日龄幼鱼,按照2℃/d速率将养殖温度分别由21.0℃和14.8℃逐步升温至27.0℃和25.0℃,记录不同家系在不同时间的死亡情况。根据热激后存活率表型筛选出高温耐受力强的20个F2家系和11尾F1亲鱼,所筛选出的亲鱼和子代家系有望成为核心育种群体构建的组成部分。To test the high-temperature tolerance of turbot,young fish of different turbot F2 families was studied through heat shock and being cultured in high temperature seawater.Experiment A：50-day post hatching（dph） young fish from F2families（year 2010） was heatshocked acutely in 30℃seawater for 2hours,then transfered into normal saewater and the survival rate was recorded for the next 48h.Experiment B：100dph youngfish from F2 families（year 2009and 2010） was reared in high temperature seawater with the water temperature being raised continuously at 2℃ /day.The survival rates of young fish in each family were recorded every 12h.Twenty F2families and 11broodstocks were selected by the survival rate phenotype post heat shock and high temperature culture.The 20F2 families and 11broodstocks of turbot with high-temperature tolerance are expected to become part of the core population for breeding.

关 键 词：大菱鲆 家系 亲本 耐高温 

分 类 号：Q89[生物学] Q958.1