牛病毒性腹泻病二价弱毒活疫苗制备工艺研究  

Study on Preparation Technique of Divalent Attenuated Virus Live Vaccine of Bovine Viral Diarrhea Virus

作　　者：李海涛[1] 苗利光[1] 刘艳环[1] 朱言柱[1] 安亚雄[1] 王坤[1] 冯卓[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《特产研究》2013年第1期1-2,18,共3页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划(20100219)

摘　　要：研究了BVDV2/JZ05-1和BVDV1/JZ05-3二价弱毒活疫苗的制备工艺。通过对BVDV2/JZ05-1和BVDV1/JZ05-3疫苗毒传代培养测定毒价后,试制出5批毒价分别是105.4TCID50/mL和105.6TCID50/mL的BVDV二价弱毒活疫苗,经检验符合试验设计要求。The aim of this study was to explore the preparation technique of divalent attenuated virus live vaccine of bovine viral diarrhea virus 2/JZ05- 1 （BVDV2/JZ05- 1） and BVDV1/JZ05- 3. BVDV2/JZ05- 1 and BVDV1/JZ05- 3 were continuously cultivated in cell culture. The poison titor was examined.5 groups of divalent attenuated BVDV live vaccine were produced. The poison prices were 10^（5.4）TCID50/mL and 10^（5.6）TCID50/mL,respectively. The vaccines were examined and was within the range of the test design requirement.

关 键 词：牛病毒性腹泻 病毒 活疫苗 

分 类 号：TQ464[化学工程—制药化工]