HPLC法测定颈康胶囊中人参皂苷Rg_1、Rb_1和三七皂苷R_1的含量  

Determination of GinsenosideRg_1,Rb_1 and Notoginsenoside R_1 in Jingkang Capsules by HPLC

作　　者：苏冰[1] 许冰[2] 赵方林 尹晶[1] 

机构地区：[1]吉林大学白求恩制药厂,长春130021 [2]吉林大学白求恩第一临床学院,长春130021 [3]吉林金柏氏食品科技有限公司,吉林白山135200

出　　处：《特产研究》2013年第1期55-56,59,共3页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划医药产业发展专项资金(YYZX201123-4)

摘　　要：建立测定颈康胶囊中人参皂苷Rg1、Rb1和三七皂苷R1含量的HPLC方法。色谱柱Alltima C18(250mm×4.6mm,5μm),流动相乙腈-水溶液,流速1.0mL/min;梯度洗脱;检测波长203nm;人参皂苷Rg1、Rb1和三七皂苷R1的线性范围0.921～6.103μg、0.634～4.387μg和0.315～2.014μg,相关系数(r)均大于0.999 0。3种皂苷的平均加样回收率在98.9%～100.9%之间,RSD均小于1.32%。本方法简便、快捷、准确,可以用于颈康胶囊的质量控制。To develop an I-IPLC method for the detemfination of active ginsenosides Rg1 ,Rb1 and Notoginsenoside Rl in Jingkang Capsules. Chromatographic separation was performed on an Alltima Cls （250mm × 4.6mm, 5μm）column; mobile phase was acetonitrile- water, flow rate was 1.0mL/min; gradient ehition; detector wavelength was 203nm. Good linear relationships of three ginsenosides were provided over investigated concentration ranges. The linear relationships of ginsenosides Rgl, Rbl and Notoginsenoside R1 were 0.921 - 6.103μg, 0.634 - 4.387μg and 0.315 - 2.014μg respectively.The values of correlation coefficient were higher than 0.999 0 for all the analytes.The average recoveries of the three components ranged from 98.9% - 100.9%. Repeatability experiments showed that relative standard deviation（RSD） values of the three contents were less than 1.32%.The developed method was simple,sensitive and reproducible,and can be used for quality control of Jingkang Capsules.

关 键 词：高效液相色谱 颈康胶囊 人参皂苷 含量测定 

分 类 号：Q503[生物学—生物化学] R927.11[医药卫生—药学]