HPLC法测定参松养心胶囊中人参皂苷Rb_1的含量  

Determination of Ginsenoside Rb_1 in Shensongyangxin Capsules by HPLC

作　　者：赵方林 苏国志[2] 尹晶[2] 许冰[3] 

机构地区：[1]吉林金柏氏食品科技有限公司,吉林白山135200 [2]吉林大学白求恩制药厂,长春130021 [3]吉林大学白求恩第一临床学院,长春130021

出　　处：《特产研究》2013年第1期57-59,共3页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划医药产业发展专项资金(YYZX201123-4)

摘　　要：建立参松养心胶囊中人参皂苷Rb1含量测定的HPLC方法。色谱柱Alltima C18(250mm×4.6mm,5μm),流动相乙腈-0.02%磷酸水溶液(32∶68),流速1.0mL/min;检测波长203nm。人参皂苷Rb1进样量在0.4～4.0μg与峰面积呈良好线性关系(r=0.999 1);平均加样回收率100.3%,RSD 1.72%。本方法结果准确,精密度及重现性较好,可以用于参松养心胶囊中人参皂苷Rb1的含量测定。To develop an HPLC method for determination of active ginsenoside Rbl in Sheasongyangxin Capsules. Chromatographic separation was performed on an Alltima Cls （250mm × 4.6mm, 5μm） column; mobile phase was acetonitrile - 0.02% phosphorie acid, flow rate was 1.0mL/min;detector wavelength was 203nm. Good linear relationships of gimenoside Rb1 was provided over investigated concentration between 0.4- 4.0/μg（ r = 0.999 1 ）. The average recoveries of ginsenoside Rb1 was 100.3 %. Repeatability experiments showed that relative standard deviation（RSD） values of ginsenoside Rb1 was 1.72 %. The developed method was acurate, sensitive and repeatable, and can be used for the deten＇nination of ginsenoside Rb1 in Shensongyangxin Capsules.

关 键 词：高效液相色谱法 参松养心胶囊 人参皂苷RB1 含量测定 

分 类 号：Q503[生物学—生物化学] R927.11[医药卫生—药学]