石油降解菌的筛选、鉴定及其菌剂制备的初步优化  

Screening and Identification of Petroleum-Degrading Microorganism and Preliminary Optimization of Bacterial Agent Preparation

作　　者：陈双喜[1] 赵阳阳[1] 邹天利[1] 张乐乐[1] 

机构地区：[1]河南大学生命科学学院生物工程研究所,河南开封475004

出　　处：《河南大学学报（自然科学版）》2013年第2期186-190,共5页Journal of Henan University:Natural Science

摘　　要：从濮阳油田石油污染土壤中筛选出20个能以原油为唯一碳源生长的菌株,对其中的优势菌KF-2进行形态学、生理生化实验及16SrRNA序列分析,鉴定为木糖氧化产碱杆菌.以KF-2为实验菌株,采用单因素和正交实验结合的方法,在150mm培养皿中研究载体种类和装量、pH、料水比、培养温度、培养时间和烘干温度对菌剂活菌数的影响,得出菌剂制备的最佳条件为:麦麸为载体、pH为9、料水比1∶3.6、10g麦麸、25℃培养3d和30℃烘干,菌剂中活菌数可达7.24×1010 CFU/g.Twenty strains with petroleum as single carbon source were isolated from petroleum contaminated soil in Puyang petroleum field. The dominant bacteria KF-2 was identified as Alcaligenes xylosoacidans through morphological, physiological and biochemical identification, as well as 16S rRNA sequence analysis. With single factor and orthogonal experiment, the effects of carrier type and amount, pH, ratio of material to water, culture temperature, culture time and dry temperature on living bacteria count of bacterial agent from KF-2 were studied on 150 mm culture dish. The optimum conditions of bacterial agent preparation were wheat bran 10 g, pH 9, ratio of material to water 1 ： 3.6, cultivation at 25 ℃ for 3 d and dried at 30 ℃. The maximum living bacteria count of KF- 2 agent attained 7.24× 1010 CFU/g.

关 键 词：石油降解 筛选 鉴定 菌剂 优化 

分 类 号：Q815[生物学—生物工程]