小麦F-box蛋白基因的电子克隆和生物信息学分析  

Electronic Clone and Bioinformatics Analysis of F-box Protein Gene from Wheat(Triticum aestivum L.)

作　　者：王俊生[1] 李俐俐[1] 杨欢[1] 谭光轩[1] 殷贵鸿[1] 

机构地区：[1]周口师范学院生命科学系,河南周口466001

出　　处：《河南农业科学》2013年第2期15-19,25,共6页Journal of Henan Agricultural Sciences

基　　金：周口师范学院大学生创新基金(2012DXS024)

摘　　要：为探讨F-box蛋白在小麦发育过程中的调节作用,利用电子克隆技术获得了一个小麦F-box蛋白基因,采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、亚细胞定位、高级结构及功能域等方面进行了预测和分析。结果表明,小麦F-box蛋白由362个氨基酸组成,在N端和C端分别存在一个F-box保守结构域和一个DUF295保守结构域(功能未知),该蛋白质二级结构中以α-螺旋(23.48%)、扩展束(25.41%)和无规则卷曲(47.24%)为主,在94-115位点处可能存在一个由内向外的跨膜区,N端不存在信号肽序列,亚细胞定位于细胞质中;该F-box蛋白与二穗短柄草、玉米和水稻的F-box蛋白相似性较高,进化距离较近。In the paper,an F-box protein gene from wheat was obtained by electronic clone technique,and some characters of the F-box protein were analyzed and predicted by bioinformatics method,including the composition of amino acid sequence, hydrophobicity, secondary structure and function of protein etc. The results showed that the F-box protein was composed of 362 amino acids,and contained an F-box domain in N end and a DUF295 conserved domain in C end of protein sequence. The secondary structure of the F-box protein mainly included a-helix （23.48%）, extended strand（25.41% ） and random coil （47.24%）. The F-box protein had higher sequece indentities and closer evolution distance with that from other Gramineae plants including Brachypodium distachyon ,Oryza sativa and Zea may.

关 键 词：小麦 F—box蛋白基因 电子克隆 生物信息学 

分 类 号：S512.1[农业科学—作物学]