LED光源不同光质下葡萄试管苗增殖和叶绿素荧光动力学特征  

Proliferation and chlorophyll fluorescence characteristics of in vitro grape plantlet with different LED irradiations

作　　者：马绍英[1,2] 李胜[1,2] 张真[1,2] 刘媛[1] 杨柯[1] 张品楠[1] 刘会杰[1] 戴彩虹[1] 

机构地区：[1]甘肃农业大学生命科学技术学院植物细胞工程研究室,甘肃兰州730070 [2]兰州汇通生物科技有限公司,甘肃兰州730000

出　　处：《甘肃农业大学学报》2013年第1期56-62,共7页Journal of Gansu Agricultural University

基　　金：国家星火计划项目(2008GA860007);甘肃省重大科技专项(1002FKDA031);甘肃省财政厅高校基本科研业务费项目(2010-N-5);甘肃省科技成果转化化项目(GNCX-2011-36)

摘　　要：采用LED不同波长(红+蓝、白、红、蓝、黄和绿)光源,以白色荧光为对照,研究不同光质条件下葡萄试管苗的增殖特性、光合特性和叶绿素荧光动力学特性.结果表明:葡萄试管苗的增殖倍数、冠鲜质量、根鲜质量、叶面积、叶绿素含量、净光合速率、叶绿荧光参数Fv/Fm、qP、ΦPSⅡ和Fv/Fo在LED红+蓝光下最高,而胡萝卜素含量和NPQ最低,在LED黄光和绿光下净光合速率为负值.在LED蓝光下,根长最短,这说明短波光抑制根系的伸长生长.The proliferation characteristics,photosynthetic characteristics and chlorophyll fluorescence dynamics characteristics of in vitro grape plantlets grown under different light quality of LED（red＋blue,white,red,blue,yellow and green）,white fluorescent as the control,were studied.The results showed that after exposure to red＋blue LED,the proliferation time,shoot fresh weight,root fresh weight,leaf area,chlorophyll content,net photosynthetic rate and chlorophyll fluorescence parameters（Fv/Fm,qP,FPSII and Fv/Fo） of the plantlets were the highest,however,the carotene content and NPQ were the lowest.The net photosynthetic rates were negative under yellow and green irradiations.The root length was the shortest with blue light irradiation.The results illustrated that short-wave light inhibited the root elongation growth.

关 键 词：葡萄试管苗 LED光质 增殖 光合作用 叶绿素荧光 

分 类 号：S663.1[农业科学—果树学]