金纳米-金属硫蛋白紫外分光光度法检测汞离子  

Ultraviolet spectrophotometry detection of Hg^(2+) using AuNPs-MTs

作　　者：王永松[1] 王永生[1] 王嘉成[1] 尹纪成[1] 钱秋梅[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《应用化工》2013年第3期552-554,共3页Applied Chemical Industry

基　　金：湖南省科技厅科技计划资助项目(2010SK3039)

摘　　要：在pH 7.0 TAE缓冲液中,金纳米、金属硫蛋白、汞离子能形成复合物,导致紫外吸收发生改变,且在5.6×10-8～2.0×10-6mol/L范围内,体系Δ(A626/A524)与汞离子的浓度有良好的线性关系,其回归方程为Δ(A626/A524)=-0.013 2+0.407 2c(×10-6mol/L),r=0.996 1,检出限为1.68×10-8 mol/L,回收率在104.3%～105.5%,该方法简单、快速、灵敏。In the tris-acetate buffer solution of pH 7.0, Hg2＋ can interact with AuNPs and MTs to form a complex, resulting in the change of ultraviolet absorbance. A （A626/A524 ） was directly proportional to the concentration of Hg2＋ in the range of 5.6×10^-8-2.0×10^-6mol/L. The equation of linear regression was A（A626/A524） = -0.013 2 ＋0.407 2c （ ×10^-6mol/L） with a correlation coefficient （r） of 0.996 1. The detection limit （LOD） was 1.68 ×10^-8 mol/L, and the recovery was 104.3% - 105.5%. The proposed method was simple ,rapid and sensitive.

关 键 词：汞 金属硫蛋白 紫外分光光度法 

分 类 号：TQ203.9[化学工程—有机化工] O657.32[理学—分析化学]