绵羊角蛋白中间丝基因KIF1.2的克隆及序列分析  

Cloning and Sequence Analysis of Ovine Keratin Intermediate Filament Gene KIF1.2

作　　者：刘鑫[1] 姜仁军[2] 王成[2] 李树伟[1,2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2013年第1期12-17,共6页Journal of Tarim University

基　　金：国家自然科学基金项目(30960272)

摘　　要：为了分析南疆地方品种羊和田羊毛囊角蛋白中间丝KIF1.2基因,以和田羊为实验动物,从绵阳毛囊中提取总RNA,利用反转录获得的cDNA第一链为模板,通过PCR扩增KIF1.2基因,插入pMD18-T克隆载体中经限制性内切酶双酶切和PCR检测双重鉴定并测定序列。结果,扩增出序列长度为1215 bp的基因片段,测序结果与GenBank上已公布的序列同源性为99%。该实验研究将为进一步通过基因和蛋白的调节来改良南疆地方品种羊和田羊羊毛品质提供必要的理论依据。In order to analyze the hair follicle keratin intermediate filaments gene KIF1.2 of south xinjiang local breed hetian sheep. the total RAN was extracted from hetian sheep hair follicles, KIF1.2 gene was amplified by PCR, then Inserted it into pMD18 -T cloning vector , digested with restriction enzyme and detected by PCR, and sequenced. The amplified fragment is 1215 bp, comparing with GenBank published sequence the homology is 99%. The experimental research will provide the necessary theoretical basis for improving the quality of the HeTian sheep by regrelation of gene and protein.

关 键 词：和田羊 KIF1.2角蛋白中间丝基因 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]