极端嗜盐古菌TRM51^T的多相分类研究  

The Polyphasic Taxonomic Research of Extreme Halophilic Archaea TRM 51^T

作　　者：任敏[1] 歹明辉[1] 夏占峰[1] 

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2013年第1期58-63,共6页Journal of Tarim University

基　　金：新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题(BRYB1002)

摘　　要：从新疆硝尔库勒盐湖分离纯化到一株极端嗜盐古菌TRM 51T,其菌落颜色为白色/粉红,细胞为杆状,能运动,革兰氏染色为阴性,生长温度范围是28～50℃(最适温度37℃),NaCl浓度生长范围是2.1～4.3 M(最适浓度为3.1 M),Mg2+生长范围是0.03～1.0 M(最适0.3 M),pH生长范围5.5～9.0(最适pH8.0)。细胞可在蒸馏水中裂解,避免细胞裂解的最低NaCl浓度为8%(w/v)。TRM 51T主要的极性脂是PG(磷脂酰甘油)、PGP-Me(磷脂酰甘油磷酸甲基酯)、TGD-2(葡糖苷甘露糖苷葡糖二醚),基于16S rDNA基因的系统发育分析显示,菌株TRM 51T与相近属Halopiger的成员相似性仅为94.2%～95.3%,与Haloterrigena类群的相似性为92.4%～94.6%,菌株TRM 51T基因组DNA的G+C mol%为63.2mol%。多相分类结果表明,菌株TRM 51T不能归入嗜盐古菌科的已描述属,建议成立新属级的新分类单元。A halophilic archaeal strain TRM511 was isolated from Xiaoerkule salt lake in Xinjiang, China. The colonies were white/ pink and the cells were rod - shaped, motile, and Gram negative. It was able to grow at 28 ～ 50℃ （ optimum 37 ℃, at 2. 1 - 4. 3 M NaC1 （optimum3. 1 MNaC1）, at0. 03-1. 0MMgC12（optimum0. 3MMgC12） and atpH5. 5-9. 0 （optimumpH8. 0）. Cells lysed in distilled water and the minimal NaC1 concentration to prevent cell - lysis was 8% （w/v）. The major polar lipids of strain TRM 51 ^T were PG （phosphatidylglycerol）, PGP -Me （phosphatidylglycerol phosphate methyl ester）, TGD -2 （glucosyl mannosyl glucosyl diether）. Phylogenetic analysis based on 16S rDNA gene revealed that strain TRM51 T was phylogenetically related to Halopiger （with 94. 2% - 95. 3% similarities） and Haloterrigena （92. 4% - 94. 6% ）. The DNA G ＋ C content of strain TRM51T is 63. 2 %. It was concluded that strain TRM51T could not he classified in any recognized genus of Archaea Branch. Strain TRM 51T is thus consid- ered to represent a novel species in a new genus within the family Halobacteriaceae.

关 键 词：极端嗜盐古菌 多相分类学 鉴定 16S RDNA基因 极性脂 

分 类 号：Q939.11[生物学—微生物学]