离子色谱法检验血液中的亚硝酸盐  

Determination of Nitrite in Blood by Ion Chromatography

作　　者：王勇[1] 左跃先[1] 宋胜利[2] 霍宗利[3] 柴锦峰 

机构地区：[1]南京市公安局刑事科学技术研究所,江苏南京210001 [2]重庆市公安局物证鉴定中心,重庆400021 [3]江苏省疾病预防控制中心,江苏南京210009 [4]山西省河津市刑警大队,山西运城043300

出　　处：《中国无机分析化学》2013年第1期80-82,共3页Chinese Journal of Inorganic Analytical Chemistry

摘　　要：建立了使用离子色谱检验血液中亚硝酸盐含量的方法。血液样品经乙腈沉淀蛋白,过DionexOnGuardⅡRP,Ag/Na前处理柱后,经IonPacAS-19阴离子色谱柱分离,用KOH淋洗液自动发生器(EG)进行梯度淋洗,抑制器采用外加水模式,电导检测器检测。NO2-标准溶液的浓度在0.214～21.4mg/L时线性关系良好,线性方程为Y=0.0209X+0.5189,相关系数r2=0.9999,血中NO2-的检出限为0.39μg/L,回收率为96.5%～101.2%。检验方法快捷,操作简便,回收率高,重现性好,可满足案件需要。A method for the determination of nitrite in blood by ion chromatography was established. The protein in blood was precipitated with aeetonitrile and then the large molecules and C1 in the supernatant were removed by using a Dionex OnGuard Ⅱ RP column and a Dionex OnGuard Ⅱ Ag/Na column. The filtrate went through gradient elution with KOH solution produced by an online eluent generator（EG）. The suppressor took the external water mode and a conductivity detector was used. When the concentration of NO2 standard solution are in the range of 0.214～21.4 mg/L, the concentration has a good linear relationship with detection signal and the linear equation is Y=0.020 9 X＋0.518 9 with r^2 =0.999 9. The limit of detection （LOD,S/N=3） of nitrite in blood was 0. 39μg/L. The average recoveries were between 96.5% to 101.2%. Because of fast testing, easy instrumental operation, high recovery, good repeatability, this method can meet the needs for public security work.

关 键 词：离子色谱法 亚硝酸盐 全血 电导检测 

分 类 号：O657.75[理学—分析化学] TH833[理学—化学]