高氧对胎鼠远端肺上皮细胞液体转运功能及上皮钠通道表达的影响  

The effect of hyperoxia on fluid transport by fetal distal lung epithelia

作　　者：纪卫华[1] 富建华[2] 薛辛东[2] 

机构地区：[1]大连市妇产医院新生儿科,116033 [2]中国医科大学附属盛京医院儿科,沈阳110004

出　　处：《国际儿科学杂志》2013年第2期201-203,207,共4页International Journal of Pediatrics

基　　金：国家自然科学基金资助项目（30872781）

摘　　要：目的探讨高氧对胎鼠远端肺上皮（FDLE）液体转运功能及上皮钠通道（ENaC）表达的影响。方法分离提取大鼠FDLE细胞，随机分成高氧组和空气组，分别在氧浓度为85％和21％的细胞培养箱中原代培养。检测高氧暴露24h和48h经FDLE细胞单层液体转运量的变化。应用Western blot方法检测高氧暴露后α-ENaC蛋白表达的变化。结果高氧48h使大鼠FDLE液体转运增加（1．78±0．19VS．1．06±0．11，P〈0．001），并且该促进作用可被阿米洛利抑制。高氧24hFDLE中α-ENaC蛋白表达与空气组比较差异无统计学意义（O．44±0．04VS．0．40±0．04，P=0．22），而高氧48hα-ENaC蛋白表达显著低于空气组（0．35±0．03VS．0．47±0．06，P=0．03）。结论高氧增强了FDLE液体转运功能，且以阿米洛利敏感性液体转运增加为主。该促进作用并非通过增加α-ENaC蛋白表达来实现。Objective To investigate the effect of hyperoxia on-fluid transport by fetal distal lung epithelia（FDLE） and the expression of epithelial sodium channel（ENaC） in these cells. Methods FDLE were isolated and randomized into hyperoxia group and normoxia group, which were primarily cultured under hyperoxic or normoxic conditions, respectively. Fluid transport was measured using monolayers of FDLE cultured on Transwell permeable inserts. Western blot was applied to examine the α-ENaC expression. Results Fluid transport across monolayer of FDLE was increased in cells exposed to hyperoxia compaired with cells cultured in normoxic conditions（ 1.78 ±0. 19 vs 1.06 ±0. 11 ,P 〈0. 001 ）. Amiloride significantly decreased the fluid transport in both of the hyperoxia and normoxia groups, but in the presence of amiloride there were no difference between the two groups. The expression of α-ENaC was inhibited by hyperoxia to some extent（ 24h ：0. 44 ± 0.04 vs 0. 40 ± 0.04,P=0.22; 48h：0.35 ±0.03 vs 0.47 ±0.06,P =0.03）. Conclusion Hyperoxia enhanced total and amiloride-sensitive fluid transport by FDLE. However,the expression of α-ENaC decreased in these cells.

关 键 词：高氧 胎鼠远端肺上皮细胞 液体转运 上皮钠通道 

分 类 号：R7[医药卫生—临床医学]