用同工酶和RAPD技术研究棉花三元杂种石远321新品种的遗传特性  

A Study of Genetic Character of Cultivar Shiyuan 321 from G. barbadense ×G. thurberi ×G. hirsutum Using Isozyme and RAPD Techniques

作　　者：刘根齐[1] 焦传珍[2] 姜茹琴[1] 张欣雪[1] 姜保功[1] 赵世民[1] 徐金相[1] 梁正兰[1] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]山东省德州高等专科学校,253023

出　　处：《Acta Genetica Sinica》2000年第11期999-1005,共7页

基　　金：中国科学院"九五"重大攻关项目!(批准号:KZ951-A1-10l-03-06)

摘　　要：石远３２１是从海岛棉×野生色伯氏棉×陆地棉三元杂种后代中选育而成的种间杂交新品种。其细胞学鉴定结果、形态学基本特征以及栽培特性均为陆地棉型。但在丰产性、棉纤维品质及抗逆性等许多农艺性状和经济性状方面，该品种明显综合了多棉种多亲本的遗传特性。为了从分子水平上认识石远３２１的三元杂种性质及其变异水平，用等电聚焦电泳和ＲＡＰＤ技术，对石远３２１及其不同棉种亲本进行了遗传分析。主要结果如下：（ｌ）酯酶同工酶图谱分析表明，石远３２１与其野生棉亲本和海岛棉亲本同具有一条陆地棉亲本所没有的特异带；（２）用４个引物对石远３２１扩增共发现６个野生棉亲本和海岛棉亲本的特征片段；（３）通过比较石远３２１与其３个不同棉种亲本之间的ＲＡＰＤ差异，说明石远３２１在基因组水平上具有高度的遗传异质性。初步从分子水平上证实了石远３２１为上述３个棉种的种间杂交后代。To study the genetic character of trispecific hybrid cultivar Shiyuan 321, we analyzed this cultivar and its parents by isozyme and RAPD techniques. The main results are as follows: (1) Esterase isozyme analysis showed that there was one specific band among cultivar Suiyuan 321 and its parents, G. thurberi and G. barbadense, (2) Six specific DNA fragments amplified by 4 primers were found among cultivar Shiyuan 321 and its parents G. barbadense, G. thurberi; (3) By comparing RAPD differences among Shiyuan 321 and its parents, it is indicated that the genetic heterogeneity of cultivar Shiyuan 321 is very high at its genomic DNA level. The above results proved that Shiyuan 321 is a trispecific cultivar from G. barbadense ×G. thurberi ×G. hirsutum as well as a new germplasm.

关 键 词：石远321 同工酶 RAPD 棉花 三元杂种 遗传特性 

分 类 号：Q943[生物学—植物学]