Immunohistochemical Analysis of IAA in Anthers of the Photoperiod-sensitive Genic Male-sterile Rice  

光敏核不育水稻花药IAA的免疫组织化学分析(英文)

作　　者：李念华[1] 童哲[1] 卢善发[1] 

机构地区：[1]中国科学院植物研究所,北京100093

出　　处：《Acta Botanica Sinica》2000年第10期1045-1050,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：PioneerFundofClimbingProgramofChina (95_2 9_0 3) ;TheNationalNaturalScienceFoundationofChina (39570 0 79)

摘　　要：The immunohistochemical localization of IAA and the comparison of their relative levels were carried out for the first time in the anthers of Nongken 58S and its wild type Nongken 58 (Oryza sativa subsp. japonica) after long_day and short_day treatments. The distribution of free_IAA in anthers and its dynamic variation could be reflected by this method. The results showed that the IAA level in the anthers of Nongken 58S after long_day treatment was much lower than that in short_day_treated Nongken 58S and those in wild type Nongken 58 in five stages from pistil and stamen primordia formation to late uninucleate stage. The possible reasons for IAA deficiency in Nongken 58S_LD anthers and its relationship with fertility alteration were also discussed.首次应用免疫组织化学分析方法对长日和短日处理后的农垦 5 8S和对照农垦 5 8(OryzasativaL .subsp .japonica)花药中的IAA进行了定位研究和相对水平的比较。结果表明 ,此方法可反映游离态IAA在花药中的分布及其相对水平的变化。从雌雄蕊原基形成期至单核晚期的 5个时期中 ,经长日照处理的农垦 5 8S花药中的IAA水平都低于短日照处理的农垦 5 8S及在不同光周期处理下的农垦 5 8花药。对花药中生长素的亏缺与育性的关系以及IAA亏缺的原因也进行了讨论。

关 键 词：rice anther auxin PHOTOPERIOD male sterility immunohistochemical localization 

分 类 号：Q945.43[生物学—植物学] Q946.885.1