诱导型一氧化氮合酶对脑外伤大鼠听功能的影响  

THE ACTION OF INDUCIBLE NITRIC OXIDE SYNTHASE ON AUDIOLOGIC FUNCTION OF DIFFUSE TRAUMATIC BRAIN INJURY IN RATS MODEL

作　　者：付兴华[1] 邓艳丽[1] 张萍[1] 张再兴[1] 卢鹤翔[2] 孙瑞军[2] 刘颖[3] 赵君晖[1] 顾洪兰[1] 韩莉[1] 

机构地区：[1]河北联合大学附属医院耳鼻咽喉科,河北省唐山市063000 [2]河北联合大学形态中心 [3]河北联合大学附属医院肿瘤科

出　　处：《中国煤炭工业医学杂志》2013年第3期430-435,共6页Chinese Journal of Coal Industry Medicine

基　　金：河北省科技厅科技支撑项目(08276101D-74)

摘　　要：目的探讨诱导型一氧化氮合酶在弥漫性脑外伤大鼠耳蜗的表达,分析诱导型一氧化氮合酶表达与听功能变化的相关性。方法建立弥漫性脑外伤大鼠模型并随机分组,采用听性脑干反应测定,光镜、免疫组织化学等手段,观察各组动物诱导型一氧化氮合酶表达及听功能的变化。结果对照组大鼠耳蜗诱导型一氧化氮合酶无表达,外伤后各组内耳诱导型一氧化氮合酶表达有明显变化(P<0.01)。外伤后各组ABRⅤ波阈值及各波潜伏期比较差异有统计学意义(P<0.05)。外伤各组内耳诱导型一氧化氮合酶表达与听功能损伤明显相关(P<0.05)。结论弥漫性脑外伤对内耳诱导型一氧化氮合酶表达及听功能均有不同程度的影响,听功能变化可能与诱导型一氧化氮合酶表达有关。Objective To explore the expression of inducible nitric oxide synthase in cochlear and auditory function on diffuse traumatic brain injury in rats. Methods Diffuse traumatic brain injury model of rats were established and randomly divided into five groups. Then auditory brainstem response examination, light microscope, immuno- histochemistical technique were used to evaluate the change of hearing function and the expression of inducible nitric oxide synthase. Results The expression of inducible nitric oxide syn- thase in the control group was normal, whereas there were obviously changes of inner ear inducible nitric oxide synthase expression on the traumatic groups. The difference of auditory brainstem response threshold and latency of wave between the experimental and the normal control group was obvious （P〈0.05）. The expression of inducible nitric oxide synthase in trauma group was significantly associated with auditory function change （P〈0.05）. Conclusion There are influence on expression of inducible nitric oxide syn- thase and auditory function in inner ear with diffuse traumatic brain injury model of rats, the decreased of auditory function may be associated with the change of inducible nitric oxide synthase expression.

关 键 词：弥漫性脑外伤 耳蜗 诱导型一氧化氮合酶 听功能 大鼠 

分 类 号：R764.83[医药卫生—耳鼻咽喉科]