舒芬太尼对大鼠肠缺血再灌注时肝组织细胞凋亡的影响  

EFFECT OF SULFENTANIL ON HEPATOCYTE APOPTOSIS INDUCED BY INTESTINAL ISCHEMIA-REPERFUSION IN RATS

作　　者：袁苑[1] 贺翠莲[1] 李佩铂[1] 

机构地区：[1]内蒙古包头市中心医院麻醉科,014040

出　　处：《中国煤炭工业医学杂志》2013年第3期439-441,共3页Chinese Journal of Coal Industry Medicine

摘　　要：目的探讨舒芬太尼对大鼠肠缺血再灌注时肝组织细胞凋亡的影响。方法成年健康Wistar大鼠24只,随机分为3组(n=8):假手术组(Sham组,S)、肠缺血再灌注组(intestine ischemical-reperfusion组,IIR)、舒芬太尼组(Sulfentanil组,SF)。通过夹闭肠系膜上动脉1h后再灌注6h制作肠缺血再灌注损伤模型,采用免疫组化法与医学图像分析检测肝组织Caspase-3蛋白表达量;TUNEL法检测肝细胞凋亡指数AI(%)。结果与S组比较,IIR组和SF组Caspase-3蛋白含量及AI均升高(P<0.05)。与IIR组比较,SF组Caspase-3蛋白含量及AI均降低(P<0.05)。结论舒芬太尼可能通过调节Caspase-3蛋白表达减轻肠缺血再灌注时肝的损伤。Objective To investigate the effects of sulfentanil on the hepatocyte apoptosis induced by intes- tinal ischemia- reperfusion in rats. Methods Twenty - four Wistar rats were randomly divided into 3 groups（n = 8） ： sham - operated control group（S group） ,intestine. ischemia - reperfusion group（IIR group） and sulfentanil group（SF group）. In the experiments, the root of superior mesenteric artery was blocked with non - injury artery clap for lh and reperfusion 6h. Expression of Caspase - 3 protein in liver tissues was detected with immunohistochemistry and MIAS- 2000. The apoptosis index （AI） of hepatic cell was measured by terminal deoxynucleotidy transferase mediated dUTP nick end labeling （TUNEL） method. Results Expression of Caspase- 3 protein of hepatic cell and AI in IIR group and SF group were signifi- cantly higher than in Sham group（P〈0.05）. Caspase- 3 protein of hepatic cell and AI in SF group were lower than in ⅡR group（P〈0. 05）. Conclusion Sulfentani 1 can relieve the liver injury by regulating Caspase- 3 protein expression.

关 键 词：舒芬太尼 肠缺血再灌注 肝细胞凋亡 大鼠 

分 类 号：R965[医药卫生—药理学]