菊花江蓠(Gracilaria lichenoides)对N、P吸收效应及其细胞超微结构变化  

UPTAKE EFFECTS OF NITROGEN AND PHOSPHORUS AND CELL ULTRASTRUCTURE CHANGES OF GRACILARIA LICHENOIDES

作　　者：黄鹤忠[1,2] 梁建生[1] 张群英[2] 

机构地区：[1]扬州大学生物科学与技术学院,扬州225009 [2]苏州大学基础医学与生物科学学院,苏州215123

出　　处：《海洋与湖沼》2013年第1期95-102,共8页Oceanologia Et Limnologia Sinica

基　　金：江苏省社会发展基金资助项目;BS2002016号

摘　　要：通过设置5个N/P浓度梯度和3个N浓度下的4个N:P梯度,进行了江蓠对N、P的短期(0—4h)和长期(第14天)吸收速率(SNUR和LNUR)以及短期和长期吸收效率(SNUE和LNUE)的研究,同时观察了5个N/P浓度梯度对江蓠细胞超微结构的影响。结果表明:N:P比为16:1时,江蓠对N、P的SNUR和LNUR均最大(P<0.05);N/P为1200/75和960/60μmol/L浓度时,分别对N和P的SNUR最大,达12.85和0.79μmol/(gDW·h),但此N/P浓度下长期培养的江蓠叶绿体结构存在不同程度的损伤;而N/P浓度480/30μmol/L时,对N和P的LNUR均最大值4.23和0.31μmol/(gDW·h)。江蓠对N、P的SNUE和LNUE在介质N/P60/3.75μmol/L时为最高,而在1200/75μmol/L时为最低(P<0.05)。The effects of variable 5 N/P concentration levels and 4 N ： P ratio levels of 3 N concentration levels, re- spectively, on uptake rates （SNUR and LNUR） and uptake efficiency （SNUE and LNUE） of N （TIN） and P （ PO3- -P） by Gracilaria lichenoides for short-term （0--4h） and long-term （over a 24h period at 14th day） were studied. Meanwhile, ul- trastructure of cells of G. lichenoides cultivated in different N and P concentration for 14 day was observed. The results showed that optimal N ： P ratio in culture media which SNUR and LNUR for N and P by G. lichenoides reached the highest value among different N/P concentrations （P〈0.05） was 16 ： 1. The reached maximum SNUR of N and P by G. lichenoides was respectively 12.85 and 0.79μmol/（gDW.h）, when the N/P concentration in medium was 1200/75 and 960/60μmol/L, but the ultrastructure of chloroplasts of G lichenoides cultivated in those N/P concentration for 14 day emerged some extent damage. The reached maximum LNUR of N and P by G lichenoides were 4.23 and 0.31 μmol/（gDW.h）, respectively, when the N/P concentration in medium was 480/30μmol/L. The reached maximum SNUE and LNUE of N and P by G lichenoides was in the medium of N/P 60/3.75μmol/L, but decreased to the minimum SNUE and LNUE, respectively, was in the medium of N/P 1200/75μmol/L.

关 键 词：江蓠 氮 磷 吸收效应 超微结构 

分 类 号：Q948.885.3[生物学—植物学]