无乳链球菌(Streptococcus agalactiae)荚膜多糖合成基因研究进展  

A REVIEW ON THE CAPSULAR POLYSACCHARIDE SYNTHESIS GENES OF STREPTOCOCCUS AGALACTIAE

作　　者：汪开毓[1,2] 黄锦炉[1] 肖丹 王均[1] 黄凌远[1] 

机构地区：[1]四川农业大学鱼病研究中心雅安625014 [2]动物疫病与人类健康四川省重点实验室雅安625014 [3]通威股份有限公司成都610041

出　　处：《海洋与湖沼》2013年第1期189-197,共9页Oceanologia Et Limnologia Sinica

基　　金：教育部“长江学者和创新团队发展计划”创新团队项目,IRT0848号;通威股份有限公司重点资助项目,2006-2009

摘　　要：无乳链球菌是一种人畜鱼共患的重要病原菌,菌体表面的荚膜多糖是公认的毒力因子,其合成过程受荚膜多糖合成基因的调控。荚膜多糖合成基因存在于荚膜多糖操纵子中,参与无乳链球菌荚膜多糖的合成启动、寡糖和多糖的聚合以及外输并锚定于菌体表面,在新型诊断技术和减毒突变株的构建方面取得良好的应用。本文首次就cps基因的基本属性、转录调节、编码蛋白及其生物功能、对荚膜多糖合成的调控机理、在血清分型和突变株构建的应用这六个方面进行深入分析和讨论,以期为GBScps基因的新功能研究和创新应用提供理论参考。Streptococcus agalactiae is an important zoonotic pathogen in human, animal and fish. The capsular poly- saccharides on the cell surface have been recognized as virulence factors, of which the forming processes are regulated by the capsular polysaccharide biosynthesis （cps） genes. The capsular polysaccharide biosynthesis genes exist in capsular polysaccharide operon of S. agalactiae genome, which were predicted to participate in the origination of capsular poly- saccharide synthesis, polymerization of oligosaccharides and polysaccharides, transport and anchor the product to the cell surface. Therefore, the cps genes achieve new applications of diagnostic techniques and the construction of attenuated mu- tant strains. In this paper, the basic properties of cps genes, as well as their transcription regulation, coding proteins and biological functions, regulation mechanism of capsular polysaccharide biosynthesis and serotyping, and application of construction of mutant strains are analyzed and discussed. We looked forward to providing a theoretical reference on the new functions research and innovative applications of S. agalactiae cps genes.

关 键 词：无乳链球菌 荚膜多糖合成基因 作用机制 应用 

分 类 号：Q7[生物学—分子生物学]