纳米低温保护剂溶液冻结和融化过程的显微研究  

A MICROSCOPIC STUDY ON THE FREEZING AND THAWING OF CRYOPROTECTANTS WITH NANOPARTICLES

作　　者：吕福扣[1] 刘宝林[1] 李维杰[1] 吴酉芝[1] 

机构地区：[1]上海理工大学生物热科学研究所上海200093

出　　处：《低温物理学报》2013年第2期155-160,共6页Low Temperature Physical Letters

基　　金：国家自然科学基金(批准号:51076108);教育部新世纪优秀人才计划(批准号:07-0559);上海市东方学者计划资助的课题~~

摘　　要：本文利用低温显微镜研究了含有不同粒径(20nm、40nm、60nm)浓度为0.5%HA纳米微粒的丙三醇溶液(40%,w/w)在不同温度变化速率(5℃/min、10℃/min、15℃/min)下的冻结和融化过程.试验结果表明:随着温度变化速率的增大,含60nm纳米微粒的丙三醇溶液,冰晶成核温度从-40℃降低到-50℃;含20nm纳米微粒的丙三醇溶液,冰晶融化起始温度从-70℃升高到-60℃.对于含40nm纳米微粒的丙三醇溶液,温度变化速率为10℃/min的冰晶属性差异显著.相比未添加、添加20nm和40nm纳米微粒的丙三醇溶液,添加60nm纳米微粒的丙三醇溶液无溶质分子淀析现象.The freezing and thawing of glycerol （40%）solutions with HA nanoparticles of different particle size （20nm, 40nm, 60nm） and concentrations（0. 5 %） were observed under a cryomicroscope at different cooling and war- ming rate（5 ℃/min, 10℃/min, 15 ℃/min）. Experimental results showed that the nucleation temperature of glycerol solution containing 60nm HA decreased from -40℃--50℃, and the incipient melting temperature of glycerol so- lution containing 20nm HA increased from --70℃- 60℃ with the speed-up of temperature change. The ice habits of glycerol solution containing 40nm HA at the velocity of 10℃/min is different remarkably. The glycerol solution containing 60nm HA has no precipitation of solute molecules when comparing with the others.

关 键 词：低温显微镜 成核温度 融化温度 淀析 

分 类 号：Q-3[生物学]