物理损伤与基质细胞衍生因子-1α仪对大鼠心肌细胞增殖和细胞因子表达的影响  

Effects of mechanical injury and stromal cell-derived factor-1α on rat cardiomyocytes proliferationand cytokine expression

作　　者：侯传举[1] 齐岩梅[1] 张端珍[1] 王琦光[1] 崔春生[1] 匡丽[1] 陈兵[1] 

机构地区：[1]沈阳军区总医院先天心内科, 沈阳110840

出　　处：《中华生物医学工程杂志》2012年第6期431-436,共6页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金（30972956）

摘　　要：目的探索物理损伤与基质细胞衍生因子1α（stromal cell—derived factor-1α，SDF-1α）对大鼠心肌细胞增殖和细胞因子表达的影响。方法培养大鼠心肌细胞，采用刮伤法建立物理损伤模型，添加80μ/L SDF-1α进行联合培养；BrdU法检测各组心肌细胞的增殖能力；碘化丙啶（PI）染色联合流式细胞仪检测各组心肌细胞周期变化；免疫细胞化学法和Western印迹方法检测心肌细胞白细胞介素1α（IL-1α）、白细胞介素6（IL-6）和转化生长因子β1（TGF—β1）的表达情况。结果SDF-1α可提高物理损伤下心肌细胞的增殖能力，同时增加细胞周期中s期所占比例；物理损伤可增加心肌细胞IL-1α（1比2．53±0．54）、IL-6（1比1．47±0．12）和TGF-β1（1比1．36±0．09）的表达，物理损伤联合SDF．1et培养组心肌细胞IL-1α和IL-6的蛋白表达量有所增加（1比1．47±0．13，1比1．54±0．17）、TGF—B1的表达却略有下降（1比0．66±0．13）。结论SDF-1α可能通过上调细胞周期中s期所占比例，促进心肌细胞增殖，并可增加物理损伤引起的促炎作用。Objective To investigate the effects of mechanical injury and stromal cell-derived factor- lcx （SDF--1α） on rat cardiomyocytes proliferation and cytokine expression. Methods Rat cardiomyocytes were cultured followed by mechanical injury by scratching and were treated with 80 txg/L SDF-1α during incubation. The proliferative capacity of cardiomyocytes was assessed by BrdU assay and changes in cell cycles were examined by PI staining and fow cytometry. Immunocytochemistry and Western blot were employed to determine the expression of interieukin （IL）-1α, IL-6 and transforming growth factor （TGF）-β1. Results SDF-1α significantly stimulated the growth of mechanically injured cardiomyocytes and increased the proportion of ceils in phase S. Mechanical injury promoted the expression of IL-1α （ 1 vs 2.53±0.54）, IL-6（1 vs 1.47±0.12） and TGF-β1 （1 vs 1.36±0.09） in cardiomyocytes. The combination of mechanical injury and SDF-1α resulted in up-regulated IL-1α and IL-6 （1 vs 1.47 ±0.13, 1 vs 1.54±0.17） expression yet slightly suppressed TGF-β1expression（l vs 0.66±0.13）. Conclusion SDF-la may promote proliferation by increasing the proportion of cells in phase S and augment mechanical injury induced proinflammatory responses in cardiomyocytes.

关 键 词：肌细胞 心脏 趋化因子CXCL12 白细胞介素类 

分 类 号：R541[医药卫生—心血管疾病]