miR125a对胆管癌细胞株QBC939增殖、凋亡影响及可能机制  

Effects of miR125a on cell cycle and apoptosis of cholangiocarcinoma cell line QBC939 and its mechanism

作　　者：汪正广[1] 徐阿曼[1] 朱亮[1] 祁义军[1] 李团结[1] 孟翔凌[1] 赵文娣[2] 

机构地区：[1]安徽医科大学第一附属医院普外科,安徽合肥230022 [2]安徽医科大学第一附属医院病理科,安徽合肥230022

出　　处：《中国药理学通报》2012年第10期1428-1431,共4页Chinese Pharmacological Bulletin

基　　金：安徽省高校省级科学研究项目(No KJ2011Z210)

摘　　要：目的探讨miR125a对胆管癌细胞株QBC939细胞增殖及凋亡的影响及可能机制。方法实时荧光定量PCR检测52例经手术和病理证实的肝外胆管癌,31例癌旁组织及10例正常胆管组织标本miR125a的表达,免疫组化检测MMP-9蛋白表达。miR125a转染胆管癌细胞株QBC939,MTT法检测细胞增殖,流式细胞分析法检测细胞周期,Caspase-3试剂盒检测凋亡,Western blot检测MMP-9蛋白表达。结果胆管癌组织miR125a表达明显下调,低于正常胆管组织(P<0.05),MMP-9明显高于正常胆管组织(P<0.05)。与对照质粒组比较,转染miR125a胆管癌细胞QBC939增殖受到抑制,流式细胞分析法表明转染miR125a的胆管癌细胞QBC939导致G1阻滞,Caspase-3试剂盒检测显示凋亡增加,Western blot分析表明转染miR125a的胆管癌细胞QBC939中MMP-9的表达出现明显的下调。结论miR125a在肝外胆管癌中低表达,它们可能与胆管癌的发生有关。胆管癌细胞株QBC939 miR125a表达上调,能够抑制细胞增殖,诱导细胞凋亡和G1阻滞,miR125a的作用可能与MMP-9的表达相关。Aim To explore the effects of miR125a on cell cycle and apoptosis inducing of cholangiocarcinoma cell line QBC939 and its mechanism.Methods The expression of miR125a was detected by real-time PCR on 52 specimens of extrahepatic cholangiocarcinoma and 10 normal bile duct.MMP-9 was assayed by immunohistochemistry.Expression plasmid of miR125a was transiently transfected into human cholangiocarcinoma cell line QBC939 and then proliferation was measured by MTT method.Cell cycle was analyzed with flow cytometry.MMP-9 expression was measured by Western blot.Results Compared with normal bile duct,the relative expression ratio of miR125a in extrahepatic cholangiocarcinoma was decreased and MMP-9 expression was up-regulated.Compared to control group,the proliferation of miR125a transfected cholangiocarcinoma cell was effectively inhibited and miR125a induced G1 phase arrest.MMP-9 expression was increased in miR125a transfected cholangiocarcinoma cells.Conclusions The expression of miR125a is down-regulated in extrahepatic cholangiocarcinoma,and it may be correlated with tumorigenesis.Up-regulation of miR125a in cholangiocarcinoma cell line QBC939 inhibits cell proliferation,arrests cell cycle which may be associated with MMP-9 expression.

关 键 词：miR125a 胆管癌 增殖 基质金属蛋白酶 凋亡 阻滞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]