腹主动脉狭窄致大鼠左室肥大后心脏射血功能及神经肽Y受体亚型变化  

Changes in ejection fraction and subtypes of neural peptide Y receptor in rats with myocardial hypertrophy induced by abdominal aortic constriction

作　　者：董颀[1] 戴文军[2] 赵路宁[3] 杨春涛[1] 陈爱兰[4] 陈敏生[5] 

机构地区：[1]广州医学院生理教研室,广东广州510182 [2]广州医学院第三附属医院心内科,广东广州510150 [3]广州医学院实验中心,广东广州510182 [4]广州医学院第一附属医院心内科,广东广州510120 [5]南方医科大学,广东广州510515

出　　处：《广州医学院学报》2012年第6期1-5,共5页Academic Journal of Guangzhou Medical College

基　　金：广东省医学科研课题(A2009246);广东省自然科学基金(S2011010004269);国家自然科学基金(30570759)

摘　　要：目的:观察压力负荷型心肌肥大大鼠左室射血功能及其神经肽Y(NPY)受体亚型(Y_1、Y_2、Y_4和Y_5)的表达变化。方法:腹主动脉缩窄法建立大鼠压力过负荷心肌肥大模型,测量全心和左心室的重量;颈总动脉插管检测左心室血流动力学;IE33彩超仪测量左室壁厚度、内径、容积,以及左室射血功能相关指标;荧光定量PCR法检测左心室组织匀浆中NPY各受体亚型mRNA的表达。结果:与假手术组相比,腹主动脉缩窄大鼠全心/体重、左室/右室均明显增高(P<0.05),左室内压力峰值(LVSP)、左室等容收缩末期压力上升最大速率(+dsp/dt_(max))和左室舒张期压力下降最大速率(-ddp/dt_(max))明显增高(P<0.05)。M型超声心动图显示,腹主动脉缩窄大鼠舒张末期室间隔厚度(IVSd)、收缩末期室间隔厚度(IVSs)、舒张末期左室后壁厚度(LVPWd)以及收缩末期左室后壁厚度(LVPWs)也较假手术组显著增加(P<0.05);通过超声推算出的左室质量,与实际测得的数值高度相关(r=0.997,P<0.05);心输出量(CO)下降至假手术组的55%(P<0.01)。另外,与假手术组相比,压力负荷型肥大心肌Y_1、Y_2、Y_4和Y_5受体的表达均显著减少(P<0.05)。结论:压力负荷导致左心室明显肥厚,收缩和舒张功能增强但心输出量降低;肥厚心肌中NPY受体各亚型表达量均显著降低。Objective： To assess the changes in left ventricular ejection fraction and neural peptide Y （NPY） receptor subtypes （Yl, Y2, Y4 and Y5 ） in pressure-overload rats with myocardial hypertrophy. Methods： Pressure-overload cardiac hypertrophy model was established by abdominal aortic constriction （AAC） in rats for assessment of the weight of the heart and left ventricle. Left ventricular hemodynamies was measured by common carotid artery catheterization. IE33 eehoeardiography was employed to determine the thickness ofvascular wall, inner caliber, volume and ejection functions of the left ventricle. The mRNA expression of NPY receptor subtypes in left ventricular tissue homogenates was detected by means of quantitative polymerase chain reaction. Results： Compare to sham group, the AAC rats yielded considerably increased ratio of heart weight to body weight and that of left ventricular weight to right ventricular weight （ both P 〈 0.05 ）. This was in line with the increased left ventricular systolic pressure （LVSP）, maximum rising rate of isovolumic systolic pressure （ ＋ dsp / dtmax ） and maximum reducing rate of diastolic pressure （ -ddp/dtmax ） （ all P 〈 0.05 ）. The M-mode echocardiography suggested that the interventricular septum diastole （IVSD）, interventricular septum systole （IVSS）, left ventricular posterior wall diastole （LVPWD） and left ventricular posterior wall systole （LVPWS） were significantly increased in AAC rats compared with sham group （ all P 〈 0.05 ）. The left ventricular mass calculated by ultrasonography was highly correlated with the measured values （ r = 0. 997 ）. AAC rats were featured by reduced cardiac output by 55% compared with sham group （ P 〈 0.01 ）. Additionally, the mRNA expression of YL, Yz, Y4, and Ys myocardial receptors in AAC rats was markedly attenuated compared with sham group （ all P 〈 0.05 ）. Conelusion ： Pressure overload results in significant cardiac hypertrophy, enhanced left ventricular sys

关 键 词：心肌肥大 神经肽Y 受体亚型 超声心动图 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]