2型糖尿病患者TCF7L2基因rsl1196218、rs290487位点多态性与代谢综合征的相关性  

Association of rs11196218, rs290487 polymorphisms in TCF7L2 gene with metabolic syndrome in type 2diabetes mellitus population

作　　者：潘蕊[1] 于金玲[1] 李晓[1] 迟爽[1] 乔虹[1] 

机构地区：[1]哈尔滨医科大学附属第二医院地方病科,150086

出　　处：《中华地方病学杂志》2013年第2期173-176,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81172742）;哈尔滨市创新人才项目（2008RFQXS092）;哈尔滨医科大学附属二院博士启动基金（BS2008-06）

摘　　要：目的探讨2型糖尿病患者中TCF7L2基因rslll96218、rs290487位点多态性与代谢综合征的相关性。方法选择2型糖尿病患者680例，按国际糖尿病联盟（IDF）诊断标准分为代谢综合征（MS）组和非代谢综合征（对照）组。提取受试者外周血DNA，PCR特异性扩增TCF7L2基因片段，通过连接酶反应，测定该基因rslll96218、rs290487位点的多态性；采用SPSS13．0软件进行统计分析。结果各群体符合Hardy—Weinberg遗传平衡，具有人群代表性；MS组TCF7L2基因rslll96218位点基因型频率GG为55．6％（233／419）、AG为35．8％（150／419）、AA为8．6％（36／419），等位基因频率G为73．5％（616／838）、A为26．5％（222／838）；rs290487位点基因型频率GG为14．8％（62／418）、AG为423％（177／418）、AA为42．9％（179／418），等位基因频率G为36．O％（301／836）、A为64．0％（535／836）。对照组TCF7L2基因rslll96218位点基因型频率GG为54．8％（126／230）、AG为39．1％（90／230）、AA为6．1％（14／230），等位基因频率G为74．3％（342／460）、A为25．7％（118／460）；rs290487位点基因型频率GG为15．0％（34／226）、AG为48．2％（109／226）、AA为36．8％（83／226），等位基因频率G为39．1％（177／452）、A为60．9％（275／452）。两组TCF7L2基因rslll96218、rs290487位点的基因型和等位基因频率比较，差异无统计学意义（P均〉0．05）。结论2型糖尿病患者中TCF7L2基因rslll96218、rs290487位点多态性与代谢综合征未见明显相关性。Objective To study the association of TCF7L2 gene rs11196218, rs290487 polymorphisms with metabolic syndrome in type 2 diabetes mellitus population. Methods According to the diagnostic criteria of international diabetes federation （IDF）, 680 cases of type 2 diabetes patients were divided into metabolic syndrome （MS） group and non metabolic syndrome （control） group. DNA was extracted from. peripheral mononuclear cells, and then PCR was performed to specifically amplify TCF7L2 gene fragments. Gene polymorphisms were determined by connected enzyme detection reaction. After population representative was checked by Hardy-Weinberg equilibrium, statistical analysis was completed by software SPSS 13.0. Results The population was accorded with Hardy-Weinberg equilibrium and possessed the population representative. Frequency distributions of genotypes（GG, AG and AA） in TCF7L2 gene m11196218 in MS and control groups were 55.6% （233/419）, 35.8% （150/419）, 8.6% （36/419） and 54.8% （126/230）, 39.1% （90/230）, 6.1% （14/230）, respectively. Frequency distributions of alleles（G and A） in TCF7L2 gene rs11196218 in MS and control groups were 73.5%（616/838）, 26.5%（222/838） and 74.3%（342/460）, 25.7%（118/460）, respectively. Frequency distributions of genotypes （GG, AG and AA） in TCFTL2 gene rs290487 in MS and control groups were 14.8%（62/418）, 42.3%（177/418）, 42.9%（179/418） and 15.0%（34/226）, 48.2%（109/226）, 36.8%（83/226）, respectively. Frequency distributions of alleles（G and A） inTCF7L2 gene rs11196218 in MS and control groups were 36.0% （301/836）, 64.0% （535/836） and 39.1% （177/452）, 60.9% （275/452）, respectively. Frequency distribution of allele and genotype in TCF7L2 genes rsl 1196218 and rs290487 between the two groups were not associated with metabolic syndrome in type 2 diabetes population （P 〉 0.05）. Conclusions TCF7L2 gene rsl 1196218, rs290487 polymorphisms has not association with metabolic syndrome of type 2 diab

关 键 词：2型糖尿病 代谢综合征 TCF7L2 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]