二斑叶螨对螺螨酯的抗性选育及其解毒酶活性测定  

Determination of selection for resistance to spirodiclofen and its effect on detoxification enzymes in Tetranychus urticae

作　　者：李忠洲[1] 周玉书[1] 朴静子[1] 田如海[1] 高萍[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866

出　　处：《应用昆虫学报》2013年第2期454-459,共6页Chinese Journal of Applied Entomology

基　　金：辽宁省教育厅项目(2009S094)

摘　　要：用螺螨酯对二斑叶螨Tetranychus urticae Koch进行35代抗性选育,抗性指数达到8.34,并在选育过程中,对二斑叶螨解毒酶的活性进行测定。结果表明,随着选育代数的增加,抗性增强,细胞色素P450活性亦显著提高,而羧酸酯酶、谷胱甘肽转移酶活性较敏感品系差异不显著。说明二斑叶螨对螺螨酯抗药性的增强与其体内细胞色素P450活性的增强有关。Selection of the two-spotted spider mite （ Tetranychus urtica Koch） for resistance to spirodiclofen and its effect on detoxification enzymes were investigated. After 35 generations of continuous selection, resistance to spirodielofen was 〉 8.34 that of the original susceptible strain. O-demethlyase activity of the F35 generation was significantly higher than that of the Fo generation. There was, however, no obvious difference in glutathione S-transferase or earboxy lesterase activity between the resistant and susceptible strains. We conclude that the resistance of T. urticae to spirodielofen is related to enhancement of O-demethlyase activity.

关 键 词：二斑叶螨 螺螨酯 抗药性 解毒酶活性 杀螨剂 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]