孕期尼古丁暴露致子代大鼠中枢对血管紧张素Ⅱ的高反应性  

Prenatal nicotine exposure induces offspring high response to central angiotensin Ⅱ

作　　者：俞峰[1] 张建国[1] 高喜仁[1] 董伟家[1] 张志智[1] 张博[1] 夏志恒[1] 张于娟[2] 

机构地区：[1]湖州师范学院医学院,浙江湖州313000 [2]苏州大学医学部实验中心,江苏苏州215123

出　　处：《解剖学报》2013年第2期157-162,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(81200482)

摘　　要：目的探讨孕期尼古丁暴露后,子代大鼠中枢对血管紧张素Ⅱ(AngⅡ)的反应性。方法对孕期尼古丁暴露的子代大鼠(n=7)脑室内注射AngⅡ、洛沙坦(Los)和PD123319(PD)后,观察尼古丁子代(尼古丁组)平均动脉压(MAP)、血气与正常大鼠子代(对照组,n=7)之间的差异,并检测下丘脑前区(AHA)c-Fos表达,AngⅡ受体1a(AT1aR)、AT1bR和AT2R mRNA的变化,以及AHA区AT1R、AT2R蛋白表达水平。结果尼古丁组基础MAP与对照组无差异,但脑室内注射AngⅡ后,尼古丁组大鼠MAP高于对照组[(120.36±6.23)mmHg比(109.87±6.86)mmHg,P<0.05],AHA区的c-Fos表达也明显强于对照组(25.8±2.91比6.42±1.52,P<0.05),而Los预处理后,两组MAP无明显变化,但PD预处理后,尼古丁组MAP仍高于对照组。并且尼古丁组AHA区的AT1aR mRNA高于对照组(1.23±0.05比1.00,P<0.05),AT1R蛋白表达也高于对照组(0.581±0.06比0.353±0.05,P<0.05)。结论孕期尼古丁暴露可导致子代大鼠AHA区AT1aR mRNA及AT1R蛋白高于对照组,可能导致中枢对AngⅡ的反应性增强。Objective To investigate the effect of prenatal nicotine exposure to the adult offspring response to central angiotensin. Methods The mean arterial pressure （MAP） and blood gas analysis after intracerebroventricular （ ICV ） injection of angiotensin II（ AngII）, losartan（Los） , PD123319 （PD） to nicotine offspring（ nicotine, n = 7 ） and control offspring（ control,n = 7） were tested. The angiotensin Ⅱ receptor 1 （ATlaR） , ATlbR and AT2R mRNA, c-Fos protein, and AT1R, AT2R protein expression of anterior hypothalamic area （AHA） were detected. Results There was no difference between the control and nicotine groups, but after ICV AngII, the MAP was significantly higher in nicotine group than in the control group [ （ 120.36 ± 6. 23 ） mmHg vs （ 109. 87 ± 6.86 ） mmHg, P 〈 0.05 ]. ICV Los in advance blocked the effect whereas ICV PD did not. The c-Fos expression was stronger in the nicotine group than that in the control group （25.8 ±2.91 vs6.42±1.52,P〈0.05）. The ATlaR mRNA（1. 23±0. 05 vs 1.00,P〈0.05）and AT1R protein（0.581 ± 0. 06 vs 0. 353± 0.05,P 〈 0. 05 ） expression were higher in the nicotine group than that in the control group. Conclusion Prenatal nicotine exposure induces higher expression of ATlaR mRNA and AT1R protein, which may result in high response to central angiotensin Ⅱ of adult offspring.

关 键 词：孕期 尼古丁 下丘脑前区 高血压 脑室插管 免疫组织化学 大鼠 

分 类 号：R331[医药卫生—人体生理学]