不同被捕食细菌对新疆盐碱地粘细菌分离的影响  

Influence of different prey strains on isolation of myxobacteria in saline-alkaline soils of Xinjiang

作　　者：李百元[1,2,3] 谢小林[2] 张鲜娇[2] 蔡卓平[2] 朱红惠[2] 

机构地区：[1]中国科学院南海海洋研究所,广州510301 [2]广东省微生物研究所,广东省菌种保藏与应用重点试验室,广东省微生物应用新技术公共试验室,广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广州510070 [3]中国科学院研究生院,北京100049

出　　处：《微生物学报》2013年第4期379-389,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31170009);新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题重点项目(BRZD1004)

摘　　要：【目的】利用被捕食细菌诱导粘细菌形成子实体的方法分离新疆盐碱地粘细菌,并初步分析被捕食菌与被诱导粘细菌之间关系。【方法】从盐碱地分离出16株对粘细菌具有不同诱导作用的被捕食细菌,将其用以分离新疆阿克苏地区盐碱地粘细菌。【结果】对25个盐碱地土样的粘细菌进行分离,共分离到55株粘细菌,结合分子生物学手段和形态学特征,初步将其归属为粘球菌属、珊瑚球菌属、Pyxidicoccus、孢囊杆菌属和侏囊菌属及6株无法纯化的疑似粘细菌。供试的16株被捕食细菌诱导粘球菌属均具有较好效果,而Pyxidicoccus和孢囊杆菌只能由革兰氏阳性菌诱导。【结论】与传统分离方法比较,采用多种被捕食细菌分离粘细菌其出菌率明显提高,数量增加,且易观察和挑取,有利于后续纯化。不同粘细菌的捕食菌谱及捕食嗜好不同,增加被捕食细菌筛选范围可能会提高粘细菌的分离效率。本研究采用被捕食菌分离粘细菌的方法为粘细菌的分离提供了新思路与途径。[Objective]We isolated myxobacteria in saline-alkaline soils of Xinjiang using a prey-predator strategy where the prey bacteria can induce the predator myxobacteria to form visible fruiting body,and evaluated intrinsic relationships between prey and predator myxobacteria. [Methods] Sixteen bacteria with inductive effects of fruiting body formation were obtained,and then used as preys to isolate the myxobacteria. [Results] A total of 55 myxobacteria strains were isolated from 25 soil samples,which were identified to the genera of Myxococcus,Corallococcus,Pyxidicoccus,Cystobacter,and Nannocystis. Besides,6 unpurified isolates were believed to be myxobacteria. All the 16 prey bacteria had preferable inductive effects on Myxococcus spp. ,whereas Pyxidicoccus spp. and Cystobacter spp. were only induced by Gram-positive strains. [Conclusion] The prey-predator strategy provided a new and more effective way to isolate myxobacteria.

关 键 词：被捕食菌 盐碱地 粘细菌 分离 

分 类 号：Q93[生物学—微生物学]