非促分裂型人酸性成纤维细胞生长因子促小鼠烫伤愈合的研究  

Experiment Study of Non-mitogenetic Human Acidic Fibroblast Growth Factor on Improving Scald Wound Healing in Mice

作　　者：沈娟[1,2] 金小宝[2] 丁静[2] 朱家勇[2] 

机构地区：[1]广东药学院生命科学与生物制药学院,广州510006 [2]广东药学院广东省生物活性药物研究重点实验室,广州510006

出　　处：《中国生物工程杂志》2013年第3期68-73,共6页China Biotechnology

基　　金：广州市科技支撑计划项目(2010J-E411);广东省教育厅育苗计划(LYM11082)资助

摘　　要：目的:研究非促分裂型人酸性成纤维细胞生长因子(non-mitogenic human acidic fibroblastgrowth factor,nmhaFGF)在促小鼠深II度烫伤创面愈合中的作用。方法:采用大肠杆菌系统表达纯化制备nmhaFGF,细胞增殖法检测nmhaFGF生物活性,构建Balb/C小鼠深II度烫伤创面模型,分为实验组(20μg nmhaFGF/cm2创面,用PBS释稀)和空白对照组(外涂PBS),观察烫伤后1,3,7,9,14 d创面面积变化,并统计创面完全愈合所需要的时间;伤后7d,21d,取各组的伤口及周边皮肤HE及免疫组化染色(PCNA,胶原蛋白),观察创面病理组织学改变,胶原排列情况。结果:获得纯度为95.1%的nmhaFGF,有丝分裂活性较haFGF大大降低,外用nmhaFGF可促小鼠烫伤愈合,将愈合时间由17.2 d缩短至15.8 d(P<0.01);伤后7 d,nmhaFGF组创面肉芽组织生长较好,PCNA表达与空白对照组相比显著增加;21 d创面再上皮化较快,胶原蛋白排列较为整齐。结论:局部用nmhaFGF能促进小鼠深II度烫伤创面愈合,明显缩短愈合时间。Objective To investigate the influence of non-mitogenetic human acidic fibroblast growth factor （nmhaFGF） on scald wound healing in mice. Method The recombinant protein nmhaFGF was expressed in Escherichia coli and purified. Mitogenic activity of nmhaFGF was analysed by Methylthiazoletetrazolium （MTT） assay. Deep II degree scald on the back in the mice was used as model. The mice were divided into nmhaFGF group（ 20 μg nmhaFGF/cm2,PBS diluted） and control group（ PBS）. The time of wound healing was recorded, The wound area was measured at 1,3,7,9,14 d after injuring. Pathological examination with HE staining and immunohistochemistry for proliferating cell nuclear antigen （ PCNA） and collagen was observed at the 7th and 14th day after injuring. Result NmhaFGF of 95.10% was obtained. Compared to haFGF, the nmhaFGF showed the weaker activity. nmhaFGF can promote reepithelization in burn wounds. Compared with the control group, nmhaFGF group showed a shorter time （from 17.2 d to15.8 d） of wound healing （P〈0.01）. The histomorphometric results indicate that granulation tissue grew better and the expression level of PCNA is higher in nmhaFGF group at the 7th day. Epidermis grew faster and arrangement of collagen was more clean in nmhaFGF group at 21th day. Conclusion Topical application of nmhaFGF can promote reepithelization of deep II degree scald wounds and shorten the healing time of burns in mice.

关 键 词：nmhaFGF 大肠杆菌 烫伤愈合 BALB C小鼠 

分 类 号：Q812[生物学—生物工程]