复合益生菌对草鱼养殖水体水质和菌群结构的影响  

Effects of complex probiotics on water quality and microbial communities in grass carp (Ctenopharyngodon idellus) culture

作　　者：郑佳佳[1] 彭丽莎[1] 张小平[1] 孙健栋[1] 傅罗琴[1] 王一冰[1] 胡彩虹[1] 李卫芬[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310058

出　　处：《水产学报》2013年第3期457-464,共8页Journal of Fisheries of China

基　　金：国家"九七三"重点基础研究发展规划(2009CB118705);公益性行业(农业)科研专项(201203083)

摘　　要：为研究草鱼养殖水体中添加复合益生菌水质调节剂对水体水质和菌群的调节作用,实验采用氮磷等指标监测水质,采用454焦磷酸盐测序方法分析菌群结构,结果显示,处理组的氨氮、亚硝酸盐氮和总氮浓度一直低于对照组,但差异不显著;处理组硝酸盐氮浓度低于对照组,且在18 d下降了56.59%;处理组的总无机氮含量低于对照组,且在15 d下降了28.75%。处理组正磷酸盐和总磷浓度略低于对照组,无显著差异。15 d水样的454焦磷酸盐测序结果与对照组相比,处理组菌群多样性更高,厚壁菌门和变形菌门分别减少了91.21%和21.75%,拟杆菌、放线菌和蓝细菌分别增加了288%、435%和848%。在变形菌门中,α-变形杆菌和β-变形杆菌分别比对照组提高了318%和18%,γ-变形杆菌比对照组降低了78.82%。研究表明,该复合益生菌具有一定水质调控功能,且能显著改变菌群结构。This experiment was conducted to study the effects of complex probiotics on water quality and microbial community structure in grass carp culture. The results of water quality determination showed that compared with the control group, the concentration of ammonia nitrogen, nitrite nitrogen and total nitrogen in treatment group was lower in the whole experiment, respectively; On 18th d, the concentration of nitrate nitrogen in treatment group was decreased by 54.49% （P 〈 0.05 ）. On 15th d, the concentration of total inorganic nitrogen in treatment group was decreased by 28. 75% （P 〈 0. 05 ）. Orthophosphate and total phosphorus had no difference between the two groups. The 454-Pyrosequencing results showed that the composition of bacterial communities was highly variable between control and treatment groups, and microbial diversity of treatment was higher than that of control. Firmicutes and Proteobacteria decreased by 91.21% （ P 〈 0.01 ） and 21.75% （ P 〈 0.01 ） respectively. Bacteroidetes, Actinobacteria and Cyanobacteria increased by 288% （P 〈 0. 01）,435% （P 〈 0. 01） and 848% （P 〈 0. 01） respectively. Among Proteobacteria,Alphaproteobacteria and Betaproteobacteria was elevated by 318% （P 〈 0.01 ）and 18% respectively, while Gammaproteobacteria was reduced by 78.82% （ P 〈 0.01 ）.

关 键 词：草鱼 复合益生菌 水质 菌群结构 

分 类 号：S917.1[农业科学—水产科学]