激光辅助耳蜗开窗对大鼠内毛细胞带状突触的影响  

Effects of laser-assisted cochleostomy on inner hair cell ribbon synapse in rats

作　　者：叶青[1] 耿阳[2] 张先增[3] 卓双木[3] 田天捷[1] 谢树森[3] 

机构地区：[1]福建医科大学省立临床医学院,福建省立医院、福建省老年医院耳鼻咽喉科,福州350001 [2]山东省淄博市中心医院耳鼻咽喉科 [3]福建师范大学激光与光电子技术研究所,福建省光子技术重点实验室,医学光电科学与技术教育部重点实验室

出　　处：《中华耳鼻咽喉头颈外科杂志》2013年第3期237-240,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金（60878062）;卫生部科学研究基金.福建省卫生教育联合攻关计划项目（WKJ2008-2-35）;福建省自然科学基金（2011J01135）

摘　　要：目的探讨超脉冲CO2激光辅助耳蜗开窗对SD大鼠内毛细胞带状突触的影响。方法将18只SD大鼠随机数字表法分为激光手术组（按输出功率又分为2W组和5W组）、假手术组和对照组。激光手术组利用超脉冲CO2激光行耳蜗底周开窗术，分别于术前、术后2d行听性脑干反应（ABR）测试。大鼠耳蜗基底膜铺片、免疫荧光染色，激光共聚焦显微镜下观察耳蜗顶回和中回的带状突触；借助3dsMax软件对突触进行计数。结果2W组及5W组的大鼠术后ABR反应阈均较术前升高，差异具有统计学意义（t值分别为-5．65和-4．97，P值均〈0．01）。与对照组相比，5W组耳蜗中回的带状突触数目明显下降（F=17．15，P〈0．01），而5W组顶回及2W组的突触数目未见明显改变（P值均〉0．05）。结论低峰功率超脉冲CO2激光辅助耳蜗开窗术后短期内对内毛细胞带状突触数目影响不大，但高功率激光辐照可对带状突触造成损伤。Objective To investigate the effects on ribbon synapse of inner hair cells by superpulsed CO2 laser-assisted cochleostomy in SD rats. Methods Eighteen SD rats were randomly divided into the laser-assisted surgery groups （2 W group and 5 W group） , the sham-operated group and the control group. Ten of those were performed a cochleostomy using superpulsed CO2 laser with corresponding power. Auditory brainstem responses （ABR） were measured pre- and postoperatively. The ribbon synapses at apical and middle cochlear turns were observed under laser scanning confocal microscope and then were quantified with 3ds Max software. Results The postoperative ABR thresholds of the 2 W and 5 W groups were larger than the preoperative cases （t = -5.65, P 〈0. 01 ; t = -4. 97, P 〈0. 01 ）. The synapse numbers at the middle turn decreased significantly in the 5 W group （F = 17.15, P 〈 0. 01 ）, while no significant changes were noted at the apical turn（P 〉0. 05）. There was no statistical difference in the 2 W group（P 〉0. 05）. Conclusions The superpulsed CO2 laser-assisted cochleostomy with high-power is accompanied by synaptic injury, while no obvious effects by the low-power laser surgery, which might be the safe strategy to preform cochleostomy.

关 键 词：激光手术 耳蜗 诱发电位 听觉 脑干 突触 毛细胞 听觉 内 大鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科]