妊高征候选基因EDN1多态性的初步研究  

A primary study of the polymorphism of candidate-gene EDN1 of PIH

作　　者：云泓若[1] 李月琴[1] 海戎[1] 姚冬生[1] 林琳[1] 谢卫兵[1] 周天鸿[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2000年第4期28-31,共4页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的 :内皮素 - 1基因是妊高征的候选基因之一。通过研究该基因TaqⅠ位点在中国人群中的多态性 ,探讨中国人群中EDN1与妊高征的关系。方法 :根据EDN1第 4内含子中多态性片段的有关序列 ,设计相应引物 ,建立检测该多态性片段的PCR技术。结果 :2 6个样品 52个内皮素- 1基因TaqⅠ位点等位基因中T1的频率为 80 9% ,T2的频率为 19 1%。结论 :显示中国汉族人群内皮素 - 1基因中存在着TaqⅠ酶切位点多态性。Aim: To investigate the association of EDN1 gene with PIH in Chinese, the polymorphism of in EDN 1 was studied. Methods: PCR assay for detecting the gene including fragment of EDN1 was developed. The length of amplicon was 358 bp. The amplified fragments were analyzed by digestion of restriction endonuclease TaqⅠ. Results: the frequency of T1 allele is 80.9%,and the frequency of T2 allele is 19.1%. Conclusion: it is confirmed that the TaqⅠpolymorphism is associated with the endothelin-1 gene in Chinese of Han nationality. [

关 键 词：内皮素-1基因 妊高征 候选基因 EDN1 多态性 

分 类 号：Q78[生物学—分子生物学] Q987