利用PCR-RFLP分析eNOS基因exon7BanⅡ酶切位点的遗传多态性  

RFLP of BanⅡ site in exon 7 of eNOS gene of Guangdong woman population

作　　者：谢卫兵[1] 李月琴[1] 周广新[1] 林琳[1] 海戎[1] 云泓若[1] 周天鸿[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2000年第4期32-35,共4页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的 :探讨内皮型一氧化氮合酶基因多态性与妊高征的相关性。方法 :应用PCR -RFLP技术检测了 12例正常女性的内皮型一氧化氮合酶基因第 7外显子。结果 :发现 12例正常女性中有 4例的基因型是eNOS/GG ,有 8例的基因型是eNOS/GT ,未检测到eNOS/TT型的个体。等位基因A1(eNOS/G)的频率为 66 7% ,等位基因A2 (eNOS/T)的频率为 33 3%。结论 :广东女性人群存在内皮型一氧化氮合酶第 7外显子BanⅡ酶切位点遗传多态性。Aim: To explore the genetic polymorphism of a BanⅡ site in exon7 of eNOS gene of woman population in GuangDong. Methods: The exon7 of eNOS gene was analysed by using polymerase chain-reaction restrict fragment length polymorphism (PCR-RFLP) technique in 12 healthy unrelated women. Results: Four of 12 healthy unrelated women were eNOS/GG genetype and eight were eNOS/GT genetype, but eNOS/TT genetype was not detected, the frequency of A1(eNOS/G) was 66.7% while A2(eNOS/T) was 33.3%. Conclusion: Results indicated that polymorphism of BanII site existed in the healthy women population in Guangdong.[

关 键 词：ENOS基因 exon7外显子 多态性 BanhⅡ酶切位点 

分 类 号：Q78[生物学—分子生物学] Q987