血必净对大鼠多脏器功能障碍综合征炎性因子的影响  被引量:6

Study of the influence of xuebijing on inflammatory cytokine in multiple organ dysfunction syndrome in rat

在线阅读下载全文

作  者:陆璐[1] 冯小明[1] 王红义[1] 牛廷献[1] 万东君[2] 王春雨[3] 罗晓红[3] 

机构地区:[1]兰州军区兰州总医院动物实验科,兰州730050 [2]兰州军区兰州总医院干部病房,兰州730050 [3]兰州军区兰州总医院内分泌科,兰州730050

出  处:《中国比较医学杂志》2013年第3期26-30,共5页Chinese Journal of Comparative Medicine

基  金:甘肃省自然科学基金(No.1010RJZA052)

摘  要:目的探讨血必净(XBJ)对多脏器功能障碍综合征(MODS)炎性因子的影响。方法将90只Wistar大鼠随机分为对照组、模型组与XBJ组。尾静脉注射内毒素(LPS)(15μg/kg)建立MODS模型。观察各组的体征表现,用ELISA方法检测TNF-α、IL-1α、IL-4和IL-10的表达水平。结果①对照组、模型组和XBJ组的死亡率分别为:0、37%和20%;②与模型组相比XBJ可促进了TNF-α的表达,同时抑制IL-1α的表达,且TNF-α和IL-1α高表达集中在6~24 h;③模型组与XBJ组IL-4的表达量在6~12 h与对照组相比明显升高(P<0.05);模型组IL-10在6~48 h与对照组相比明显升高(P<0.05);XBJ组IL-10的表达水平在6 h与对照组相比明显升高(P<0.05)。结论 XBJ能降低LPS介导的大鼠MODS的死亡率,可同时促进TNF-α释放和抑制IL-1α、IL-4和IL-10释放,有效改善MODS的反应状态。Objective To evaluate the influence of Xuebijing (XBJ) by inflammation cytokines of multiple organ dysfunction syndrome (MODS). Methods 90 rats were randomly separated into 3 groups, the control group and XBJ group were infused 15 μg/kg LPS in vena caudalis, Then the physical sign appearance was observed and collection the serum to determine the expression concentration of TNF-α IL-1α IL-4 and IL-10 by ELISA in each group at different times. Results (1) The death rate of rats were the percent of 0, 37, and 20 in the control group,model group and XBJ group, respectively. (2) The XBJ enhance the increased amplitude of TNF-α and deduce the increased amplitude of IL-1α by compared with model group, the expression concentration peak of TNF-α and IL-1α is 6 -24 h. (3) The expression concentration of IL-4 in model group and XBJ group in model group has significant ascendant compared with control group at 6 -48 h; XBJ group has significant have significant ascendant compared with control group at 6 - 12 h (p 〈 0.05 ). The expression concentration of IL-10 ascendant compared with control group at 6h (p 〈 0. 05). Conclusion The results demonstrated that XBJ could degrade the death rate of MODS induced by LPS,and it could reduce the increased amplitude of TNF-α, whereas enhance the increased amplitude of the serum IL-1α, IL-4 and IL-10, it could also prevent those rats from changing into MODS efficiently.

关 键 词:血必净 多脏器功能障碍综合征 死亡率 炎性因子 

分 类 号:R332[医药卫生—人体生理学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象