抗骨质疏松药XW630对成骨细胞碱性磷酸酶mRNA表达的影响  

Effect of antiosteoporosis drug XW630 on osteoblast gene expression

作　　者：尚德志[1] 王大章[2] 郑虎[3] 翁玲玲[3] 邓力[3] 

机构地区：[1]锦州医学院口腔系 [2]华西医科大学口腔医学院 [3]华西医科大学药学院

出　　处：《中国骨质疏松杂志》2000年第3期1-4,共4页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目!(39430122)

摘　　要：目的　研究新设计的抗骨质疏松药物 XW630对成骨细胞碱性磷酸酶基因表达的影响 ,为其治疗骨代谢性疾病提供理论和实验依据。方法　以成骨细胞 MC3T3- E1为体外实验模型 ,用含有10 -6mol/ L浓度的 XW630 ,雌酚酮 ,四环素及四环素加雌酚酮的细胞培养液 DMEM培养细胞 ,2 4 h和72 h提取细胞总 RNA并与碱性磷酸酶 c DNA进行 Northern杂交。结果　XW630对成骨细胞 MC3T3-E1合成和分泌碱性磷酸酶具有明显的促进作用。Objective\ To find the effect of a new antiosteoporosis drug XW630 on alkaline phosphatase of gene expression and to support the theoretic and experimental basis for XW630 in treating the bone metabolic disease.Methods The osteoblasts MC3T3 E 1 were cultured in vitro with 10 -6 mol/L XW630 containing culture medium,while estrogen and tetracycline were used as control drugs in the medium.The osteoblasts were cultured for 24 hours and 72 hours,the total RNA of osteoblasts was extracted,the alkaline phosphatase was used as cDNA probe and the Northern blot hybridization was done.Results The mRNA expression of osteoblast alkaline phosphatase could be promoted by XW630.Conclusion The metabolism of osteoblasts could be promoted by XW630.

关 键 词：抗骨质疏松药 碱性磷酸酶 骨质疏松 MRNA 

分 类 号：R681.05[医药卫生—骨科学] R965[医药卫生—外科学]