类风湿关节炎和骨关节炎滑膜滑液中MMP-2和MMP-9的比较  

Comparison of the MMP-2 and MMP-9 in the Knee Joint Synovial Fluid and Synovial Membrane in Patients with active Rheumatoid Arthritis and Osteoarthritis

作　　者：刘荣清[1] 林佳静[2,3] 孙伯坚[2] 李海波[1] 宋婷阁[2] 韩梅[2] 

机构地区：[1]宁夏医科大学总医院风湿免疫科,银川750004 [2]宁夏医科大学基础医学院病原生物学与医学免疫学系,银川750004 [3]河北省沧州中西医结合医院,沧州061001

出　　处：《宁夏医科大学学报》2013年第1期23-25,F0003,共4页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81260459);宁夏自然科学基金(NZ09107)

摘　　要：目的检测基质金属蛋白酶-2(matrix metalloproteinases-2,MMP-2)和基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)在类风湿关节炎活动期(rheumatoid arthritis,RA)和骨关节炎(osteoarthritis,OA)活动期膝关节滑液及滑膜中的含量,并探讨两者在RA和OA病变中的作用。方法收集宁夏医科大学总医院风湿免疫科和骨科确诊的14例RA活动期患者和24例OA活动期患者的膝关节滑液和滑膜组织,用酶联免疫吸附实验(ELISA)检测滑液中MMP-2和MMP-9的含量,用免疫组化法检测滑膜组织中MMP-9的相对表达量,组间比较采用秩和检验。结果 RA活动期患者膝关节滑液中的MMP-2和MMP-9浓度明显高于骨关节炎活动期患者(P<0.01);RA活动期患者膝关节滑膜中MMP-9的相对表达量明显高于OA活动期患者(P<0.01)。结论 MMP-2和MMP-9在RA活动期患者滑液中高表达,MMP-9在RA活动期患者滑膜中高表达,可能参与RA发生发展过程中滑膜炎、骨质破坏。Objective To compare matrix metalloproteinases - 2 （ MMP - 2） and matrix metalloproteinases - 9 （MMP-9） in knee joint synovial fluid and synovial membrane in patients with active rheumatoid arthritis（RA） and active osteoarthritis （ OA）, and to explore the effects of MMP - 2 and MMP - 9 on lesion of RA and OA. Methods Knee joint synovial fluid and synovial membrane were collected from 14 and 24 patients withfinal diagnosis of RA and OA, and underwent surgery at the General Hospital of Ningxia Medical University. MMP -2 and MMP -9 in the synovial fluid were measured by enzyme linked immunosorbent assay （ELISA）.The distributions of MMP -9 in the synovial membrane were analyzed using immunohistochemistry. Student＇s t -test was used for intergroup comparison. Results The level of MMP -2 and MMP -9 in synovial fluid ofactive RA was significantly higher than that of active OA（ P 〈 0.01 ） ; the relative expression of MMP - 9 in synovial membrane was stronger in active RA than that in active OA（P 〈 0.01 ）. Conclusion High expres-sion of MMP - 2 and MMP - 9 in active RA synovial fluid, and high expression of MMP - 9 in active RA syno- vial membrane may suggested that MMP- 2 and MMP -9 as the remodel enzymes that participate in chronicsynovitis, bone destruction and joint malformation.

关 键 词：基质金属蛋白酶-2 基质金属蛋白酶-9 类风湿关节炎 骨关节炎 滑液 滑膜 

分 类 号：R593.22[医药卫生—内科学]