超声辐射力促靶向微泡黏附的在体实验研究  

Experiment of the ultrasound radiation force enhancement in adhesion of targeted microbubbles in vivo

作　　者：王宝平[1] 吴凤林[1] 纪丽景[1] 许婷婷[1] 

机构地区：[1]南方医科大学南方医院超声科,广州510515

出　　处：《中华超声影像学杂志》2013年第3期255-258,共4页Chinese Journal of Ultrasonography

摘　　要：目的探讨超声辐射力对靶向微泡在体黏附性能的影响。方法建立小鼠提睾肌炎症模型，经尾静脉团注脂质微泡（MB）或靶向微泡（MB_ICAM），同时进行超声辐射力（USRF）照射或假照5min。实验随机分为MB＋假照组和MB＋USRF组，MB_ICAM＋假照组和MB_ICAM ＋ USRF组。荧光显微镜下观察微泡黏附情况并计数。结果USRF组MB_ICAM在微循环中的黏附数量比假照组显著增高，分别为（43．4±2．1）／视野，（14．8±1．8）／视野，两者之间具有显著性差异（P=0．000）。USRF组MB在微血管中的黏附数量[（6．2±1．3）／视野）较假照组（4．6±0．9）／视野]有轻度增高，但两者之间差异无统计学意义（P=0．129）。MB_ICAM 黏附数量在辐射力组及假照组均显著高于MB的黏附数量。结论超声辐射力可显著提高在体微血管内靶向微泡的黏附作用。Objective To investigate the effects of ultrasound radiation force（USRF） on adhering of targeted microbubbles in vivo. Methods A rat model of cremaster muscle Inflammation was developed,and mierobubble（MB） or targeted microbubble of intercellular adhesion molecule-1 （MB_ICAM） was injected through the caudal vein. Immediately after intravenous MB or MB_ICAM injection,the cremaster was insonated for 5 minutes and the control group was in sham ultrasound exposure. All mice were divided into four groups randomly： ① MB ＋ false USRF;② MB ＋ USRF; ③MB_ICAM ＋ false USRF;④MB_ICAM ＋ USRF. Fluorescence microscope was performed in twenty mice to evaluate mierohuhhles adhesion and video recordings were made with a high-resolution camera, Results The adhesion number of MB_ICAM was significantly greater in USRF group than that of MB_ICAM in false USRF group [ （43.4± 2.1 ）/view vs （14±1.8）/view, P = 0. 000]. The adhesion number of MB in USRF group was greater than in false USRF group [（6.2 ±1.3）/view vs （4, 6 ± 0.9）/view]. But there is no statistical significance （ P = 0. 129）. The adhesion number of MB_ICAM in both USRF group and false USRF group was significantly greater than the adhesion number of MB. Conclusions The ultrasound radiation force can significantly improve the adhesion of targeted microbubbles in vivo.

关 键 词：超声检查 微气泡 胞间黏附分子-1 

分 类 号：R445.1[医药卫生—影像医学与核医学]