Ghrelin对果糖诱导大鼠高血压伴胰岛素抵抗的影响  

Effects of Ghrelin on hypertension and insulin resistance in fructose-fed rats

作　　者：赵荫涛[1] 邵莉[2] 杨海波[1] 李凌[1] 滕丽莉[2] 

机构地区：[1]郑州大学第一附属医院心血管内科,450052 [2]同济大学附属东方医院老年医学科

出　　处：《中华医学杂志》2013年第12期935-938,共4页National Medical Journal of China

基　　金：国家自然科学基金（30600294）

摘　　要：目的探讨Ghrelin治疗高血压和胰岛素抵抗的作用及机制。方法雄性sD大鼠32只，计算机随机分为正常对照组（A组，n=16）和果糖诱导组（B组，n=16）。B组10％果糖饮水共4周，建立高血压伴胰岛素抵抗大鼠模型。A组分为GA1组（n=8）给予等容积生理盐水，GA2组（n=8）给予50nmol／kgGhrelin；B组分为GB1组（n=8）给予等容积生理盐水，GB2组给予50nmol／kgGhrelin，各组均分别于每天两次腹膜内注射方式给药共持续6周。测定大鼠尾动脉压、血糖、血脂、血浆胰岛素和15-F2t-isoprostane等生化指标以及计算稳态模型胰岛素抵抗指数（HOMA-IR）。结果GB2组大鼠3周后收缩压开始出现明显下降（P〈0．05），第5周血压下降达到稳态，高于GA1组[（127±5）比（120±6）mmHg，P〈0．05，1mmHg=0．133kPa]。GB2组大鼠血浆胰岛素浓度和HOMA—IR均较GB1组明显低[（9．6±2．6）比（13．1±3．6）μU／ml，P〈0．05；1．92±0．12比2．78±0．14，P〈0．01]。GB2组血浆15-F2t-isoprostane含量明显低于GBl组[（75±11）比（102±14）pg／ml，P〈0．01]。结论Ghrelin可能通过抑制氧化应激反应降低果糖诱导大鼠血压和血浆胰岛素浓度，改善机体胰岛素抵抗。Objective To explore the effects and mechanisms of Ghrelin on hypertension and insulin resistance in fructose-fed rats. Methods A total of 32 male Sprague-Dawley （ SD ） rats were randomized into control （A） and fructose-fed groups （B）. Rats in group B were fed with 10% fructose solution for 4 weeks. Then the rats in group A were randomized into intraperitoneal saline （ group GA1 ） or intraperitoneal 50 nmoL/kg Ghrelin （ group GA2 ） and those in group B into intraperitoneal saline （ group GB1 ） or intraperitoneal 50 nmol/kg Ghrelin （group GB2） twice daily for 6 weeks. Caudal arterial pressure was measured weekly. Plasma blood glucose, insulin concentration and lipid profile were measured. And insulin resistance （IR） was calculated by the method of homeostasis model assessment （HOMA）. Plasma level of 15-F2t-isoprostane was measured by enzyme-linked immunosorbent assay （ELISA）. Results Ghrelin caused a significant reduction of systolic pressure at Week 3 in group GB2 versus group GB1 （ P 〈 0. 05 ）. Maximal effect appeared at Week 5 （ （ 127 ± 5 ） vs （ 120 ± 6 ） mm Hg, P 〈 0. 05,1 mm Hg = 0. 133 kPa） , but blood pressure failed to reach normal value. Ghrelin also decreased plasma insulin concentration andHOMA-IR （（9.6±2.6）vs（13.1±3.6）μU/ml, P〈0.05;1.92±0.12 vs2.78±0.14, P〈0.0 1）. Plasma level of 15-F2t-isoprostane was lower in group GB2 than that in group GB1 （ （75 ± 11 ） vs（ 102 ± 14） pg/ml, P 〈 0. 01 ）. Conclusion Ghrelin may lower blood pressure, ameliorate insulin resistance and improve insulin sensitivity through inhibiting oxidative stress in fructose-induced rats.

关 键 词：果糖 高血压 胰岛素抗药性 GHRELIN 大鼠 

分 类 号：R587.1[医药卫生—内分泌]