双链DNA微阵列:原理、技术和应用  

Double-stranded DNA microarray: principal, techniques and applications

作　　者：潘艳[1] 王进科[1] 

机构地区：[1]东南大学生物电子学国家重点实验室,南京210096

出　　处：《遗传》2013年第3期287-306,共20页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:61171030)资助

摘　　要：双链DNA微阵列也称为蛋白结合微阵列,是高通量检测分析DNA结合蛋白(如转录因子)与大量DNA分子相互作用的一种重要技术。它将大量双链DNA分子固定在特定的固相支持物(如玻片)上,与待测蛋白相互作用,用以确定转录因子的DNA结合亲和性、特异性及序列偏好性。近年来,该技术在快速表征大量转录因子的DNA特异性、绘制转录因子DNA结合谱、鉴定转录因子DNA结合位点和靶基因、识别转录因子家族内不同成员及其二聚体的细微DNA结合差异、考察辅助因子对转录因子DNA结合特异性影响等方面展现了其重要的应用价值。文章对双链DNA微阵列的原理、技术及应用进行了综述。Double-stranded DNA （dsDNA） microarray, also known as protein binding microarray （PBM）, is an impor- tant technique that can be used to assay the interaction of DNA-binding protein （such as transcription factor, TF） with vast amount of DNA molecules in high-throughput format. This technique immobilizes large amount of various dsDNA mole- cules on the surface of a solid support （such as glass slide） for detecting the binding interaction of a DNA-binding protein with all of the immobilized dsDNA molecules, and thus determining the DNA-binding affinity, specificity and preference of TFs. In recent years, this technique has demonstrated its valuable applications in several aspects, including rapidly charac- terizing DNA-binding specificity of large number of TFs, building DNA-binding profiles of TFs, identifying DNA-binding sites and target genes of TFs, discriminating the subtle DNA-binding preferences of members and their dimmers of a TF family, and examining the effects of a cofactor on the DNA-binding specificity of TFs. This paper reviews the principal, techniques, and applications of dsDNA microarray.

关 键 词：双链DNA微阵列 转录因子 

分 类 号：Q78[生物学—分子生物学]