不同年龄段人血清中酸性肽的HPLC分析  

HPLC analysis of acidic peptide contents in serum of persons with different ages

作　　者：谢江涛[1] 安玉会[1] 王洁琼[1] 贺司宇[1] 毛红利[1] 施佳辰[1] 徐霞[1] 

机构地区：[1]郑州大学基础医学院生物化学与分子生物学教研室,河南郑州450001

出　　处：《河南医学研究》2013年第1期1-3,共3页Henan Medical Research

摘　　要：目的:了解不同年龄人群血清中酸性肽的含量,以寻找神经退行性改变的早期预警的生物标志物。方法:采用内标法和高效液相色谱(high performance liquid chromatography,HPLC)分析法。结果:分析结果显示:1～10岁组(A组),20～30岁组(B组),40～59岁组(C组)和60～80岁组(D组)人群血清中酸性肽的含量分别为2.943±1.340、2.338±0.371、2.052±0.328、(2.778±0.499)mg/ml。A组与B组相比P=0.082,A组与C组相比P=0.012,A组与D组相比P=0.628。结论:20～30岁组和40～59岁组人群血清中酸性肽的含量比1～10岁组和60～80岁组人群血清中酸性肽的含量低,具有统计学意义。Objective ： In order to realize the contents of acidic peptide in serum of persons with different ages, so that find a biomarker to use as a predictor for early warning of nerve degeneration. Methods： The methods of inner standard agent and HPLC analysis were used. Results： Analysis results show that the contents of acidic peptide in 1-10 year group（ A group） , 20-30 year group（ B group）, 40-59 year group （ C group） and 60-80 year group （ D group） are alone 2. 943 ± 1. 340, 2. 338 ± 0.371,2. 052 ± 0. 328, （2. 778 ± 0. 499 ） mg/ml. When A group compares with B, P equals to 0. 082. While A group compares with C group, P equals 0. 012. When A group compares with D group, P equals to 0. 628. Conclusion ： The contents of acidic peptide in 20-30 year group and 30-59 year group are lower than ones in 1-10 year group and 60-80 year group, and the data have statistic significance.

关 键 词：酸性肽 人血清 含量 高效液相色谱 分析 

分 类 号：R446.1[医药卫生—诊断学]