高效液相色谱法测定复方狼毒注射液中苦参碱的含量  

Determination of Matrine in Compound Radix Euphorbiae fischerianae Injection by HPLC

作　　者：宋茹[1] 袁继民[1] 于连生 

机构地区：[1]中国人民解放军济南军区药品检验所,山东济南250022

出　　处：《时珍国医国药》2000年第9期789-790,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：采用 HPL C法 ,以 Shim-pack CL C-ODS为色谱柱 ,乙腈 -0 .0 2 mol· L- 1 磷酸二氢钠 (60∶ 40 )为流动相 ,检测波长 2 1 0 nm,测定复方狼毒注射液中苦参碱的含量。结果 :苦参碱在 0 .1 5～ 0 .90 mg· ml- 1范围内呈线性关系 ,γ=0 .9993 ,平均回收率为 99.1 3 % ,RSD=0 .99%。方法简便快速 ,结果准确可靠 ,可作为该制剂的质量控制方法。HPLC method is used for the determination of matrine in Compound radix euphorbiae fischerianae injection with the column of Shim pack CLC ODS, the mobile phase of acetonitrile 0.02 mol·L -1 sodium phosphate monobasic (60∶40), detection wavelength of 210 nm. Results: The linear range is 0.15～0.90 mg·ml -1 ,γ=0.999 3, the average recovery is 99.13% and RSD=0.99%. The method is simple, rapid and accurate, it can be a quality control standard of Compound Radix Euphorbiae fischerianae injection.

关 键 词：HPLC法 复方狼毒注射液 苦参碱 中药 

分 类 号：R284.1[医药卫生—中药学]