铕与胰蛋白酶、胃蛋白酶和血红蛋白之间的相互作用  

Interaction of Eu(Ш) with Trypsin, Pepsin and Hemoglobin

作　　者：金东海 李静[2] 李艳梅[2] 高艳芳[2] 刘进荣[2] 

机构地区：[1]内蒙古呼伦贝尔市公安局刑事科学技术研究所,021008 [2]内蒙古工业大学化工学院,呼和浩特010051

出　　处：《内蒙古工业大学学报（自然科学版）》2013年第1期44-49,共6页Journal of Inner Mongolia University of Technology：Natural Science Edition

基　　金：国家自然科学基金(50667001);"西部之光"人才培养计划

摘　　要：稀土的摄入量直接影响着肌体的免疫力,因此研究稀土离子与蛋白质之间的相互作用是一个重要的研究方向。本文采用电化学方法、紫外-可见吸收光谱法以及荧光光谱法研究了在人体生理环境下Eu3+与胰蛋白酶、胃蛋白酶、血红蛋白之间的相互作用。实验结果表明:Eu3+的可逆氧化还原峰电流随着三种蛋白质的加入量增加而降低,Eu3+与三种蛋白质相结合并形成了复合物。紫外可见吸收光谱的结果显示Eu3+稀土离子的引入改变了三种蛋白质的构象。荧光光谱表明,当使用278nm和295nm波长的光激发均出现了Trp残基的荧光发射峰,三种蛋白质的荧光强度随着Eu3+稀土离子浓度的增加而降低,说明Eu3+稀土离子使Trp残基的荧光部分发生猝灭。The intake of rare earth directly affects the body＇s immunity,so the interaction between rare earth ions and proteins is an important research direction.Interation between Eu3＋ rare earth ion and three proteins was studied electrochemical methods,UV-Vis absorption spectra and fluorescence spectra in physiological environment.The experimental results shown that the reversible redox peak current of Eu3＋ rare earth ion reduced with increasing three proteins content.Eu3＋ was combined with three proteins and obtained composite.UV-Vis absorption spectra shown that the addition of Eu3＋ changed the conformation proteins.The fluorescence spectra shown that fluorescence emission peak of Trp residue was found,when using 278nm and 295nm wavelength.The fluorescent intensity of three proteins decreased with increasing Eu3＋ concentration,this was explained that presence of Eu3＋ led to part quenching of three proteins fluorescence.

关 键 词：铕 胰蛋白酶 胃蛋白酶 血红蛋白 

分 类 号：O61[理学—无机化学]